• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Immunology: An Official Journal of the British Society for Immunology >Bright expression of CD91 identifies highly activated human dendritic cells that can be expanded by defensins
【24h】

Bright expression of CD91 identifies highly activated human dendritic cells that can be expanded by defensins

机译：CD91的明亮表达鉴定出可以被防御素扩增的高度活化的人树突状细胞

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

CD91 is a scavenger receptor expressed by different immune cells and its ligands defensins have been demonstrated to contribute to immune responses against infections and tumours. We previously demonstrated that CD91 is expressed on human monocyte-derived dendritic cells (moDCs) and that human defensins stimulate in vitro the activation of these cells. In this study, we observed that CD91 is expressed at different levels on two distinct moDC subsets: CD91(dim) and CD91(bright) moDCs. Although CD91(bright) moDCs represented a small proportion of total moDCs, this subset showed higher levels of activation and maturation markers compared with CD91(dim) moDCs. The frequency of CD91(bright) moDCs increased by similar to 50% after in vitro stimulation with recombinant human neutrophil peptide-1 (rHNP-1) and recombinant human defensin-1 (rHBD-1), while lipopolysaccharide (LPS) stimulation decreased it by similar to 35%. Both defensins up-regulated moDC expression of CD80, CD40, CD83 and HLA-DR, although to a lower extent compared with LPS. Notably, upon culture with rHNP-1 and rHBD-1, CD91(bright) moDCs maintained their higher activation/maturation status, whereas this was lost upon culture with LPS. Our findings suggest that defensins promote the differentiation into activated CD91(bright) DCs and may encourage the exploitation of the CD91/defensins axis as a novel therapeutic strategy to potentiate antimicrobial and anti-tumour immune response.
机译：CD91是由不同免疫细胞表达的清道夫受体，其配体防御素已被证明有助于抵抗感染和肿瘤的免疫反应。我们以前证明CD91在人单核细胞衍生的树突细胞（moDCs）上表达，人防御素在体外刺激这些细胞的激活。在这项研究中，我们观察到CD91在两个不同的moDC亚组上以不同的水平表达：CD91（dim）和CD91（bright）moDC。尽管CD91（亮）moDC占总moDC的一小部分，但与CD91（暗）moDC相比，该子集显示出更高水平的激活和成熟标记。重组人嗜中性粒细胞肽1（rHNP-1）和重组人防御素1（rHBD-1）体外刺激后，CD91（亮）moDC的频率增加了约50％，而脂多糖（LPS）刺激则降低了它大约是35％两种防御素均上调CD80，CD40，CD83和HLA-DR的moDC表达，尽管与LPS相比程度较低。值得注意的是，在用rHNP-1和rHBD-1培养后，CD91（亮）moDC保持了较高的激活/成熟状态，而在LPS培养后却消失了。我们的发现表明防御素可促进分化成活化的CD91（亮）DC，并可能鼓励利用CD91 /防御素轴作为增强抗微生物和抗肿瘤免疫应答的新型治疗策略。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号