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首页> 外文期刊>Immunology: An Official Journal of the British Society for Immunology >Interleukin (IL)-4 promotes T helper type 2-biased natural killer T (NKT) cell expansion, which is regulated by NKT cell-derived interferon-gamma and IL-4.
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Interleukin (IL)-4 promotes T helper type 2-biased natural killer T (NKT) cell expansion, which is regulated by NKT cell-derived interferon-gamma and IL-4.

机译：白介素（IL）-4促进T辅助2型偏向自然杀伤T（NKT）细胞的扩增，这受NKT细胞衍生的干扰素-γ和IL-4的调节。

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CD1d-restricted natural killer T (NKT) cells can rapidly produce T helper type 1 (Th1) and Th2 cytokines and also play regulatory or pathological roles in immune responses. NKT cells are able to expand when cultured with alpha-galactosylceramide (alpha-GalCer) and interleukin (IL)-2 in a CD1d-restricted manner. However, the expansion ratio of human NKT cells is variable from sample to sample. In this study, we sought to determine what factor or factors are responsible for efficient in vitro expansion of NKT cells from various inbred mouse strains. Although the proportion of NKT cells in the spleen was nearly identical in each mouse strain, the growth rates of NKT cells cultured in vitro with alpha-GalCer and IL-2 were highly variable. NKT cells from the B6C3F1 and BDF1 mouse strains expanded more than 20-fold after 4 days in culture. In contrast, NKT cells from the strain C3H/HeN did not proliferate at all. We found that cell expansion efficiency correlated with the level of IL-4 detectable in the supernatant after culture. Furthermore, we found that exogenous IL-4 augmented NKT cell proliferation early in the culture period, whereas interferon (IFN)-gamma tended to inhibit NKT cell proliferation. Thus, the ratio of production of IL-4 and IFN-gamma was important for NKT cell expansion but the absolute levels of these cytokines did not affect expansion. This finding suggests that effective expansion of NKT cells requires Th2-biased culture conditions.
机译：受CD1d限制的自然杀伤T细胞（NKT）可以快速产生T辅助1型（Th1）和Th2细胞因子，并且还可以在免疫反应中发挥调节或病理作用。当与α-半乳糖神经酰胺（α-GalCer）和白介素（IL）-2以CD1d限制性方式培养时，NKT细胞能够扩增。然而，人NKT细胞的扩增比例在样品之间是可变的。在这项研究中，我们试图确定哪些因素负责各种近交小鼠品系的NKT细胞的有效体外扩增。尽管每种小鼠品系中脾脏中NKT细胞的比例几乎相同，但体外用α-GalCer和IL-2培养的NKT细胞的生长速率却变化很大。培养4天后，来自B6C3F1和BDF1小鼠品系的NKT细胞扩增了20倍以上。相反，来自C3H / HeN菌株的NKT细胞根本不增殖。我们发现，细胞扩增效率与培养后上清液中可检测到的IL-4水平相关。此外，我们发现外源性IL-4在培养初期会增强NKT细胞的增殖，而干扰素（IFN）-γ则倾向于抑制NKT细胞的增殖。因此，IL-4和IFN-γ的产生比例对于NKT细胞扩增很重要，但是这些细胞因子的绝对水平并不影响扩增。这一发现表明，NKT细胞的有效扩增需要偏向Th2的培养条件。

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