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首页> 外文期刊>European journal of pharmaceutical sciences >Survival of different cell lines in alginate-agarose microcapsules.
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Survival of different cell lines in alginate-agarose microcapsules.

机译：在藻酸盐-琼脂糖微胶囊中不同细胞系的存活。

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Cell microencapsulation has emerged as a promising therapeutic strategy to treat a wide range of diseases. The optimisation of this technology depends on several critical issues such as the careful selection of the cell line, the controlled manufacture of microcapsules and the suitable adaptation of the construct design to the selected cell line. In this work, we studied the behavior of hybridoma cells once enclosed in solid and liquefied core alginate-agarose beads. Results show that hybridoma cells presented a better growing pattern and improved their viability and antibody production within liquefied beads. However, when these beads were evaluated with a compression resistance study, they were found to be mechanically more fragile than solid ones. To address this problem, we entrapped non-autologous cells (BHK fibroblast and C2C12 myoblast) in solid alginate-agarose beads and observed that they showed an improved growing profile and prolonged their viability up to 70 days in comparison to the 15 days seen for the hybridoma cells.
机译：细胞微囊化已经成为治疗广泛疾病的一种有前途的治疗策略。该技术的优化取决于几个关键问题，例如精心选择细胞系，控制微胶囊的生产以及构建体设计对所选细胞系的适当适应。在这项工作中，我们研究了一旦被固体和液化的核心藻酸盐-琼脂糖珠包裹，杂交瘤细胞的行为。结果表明，杂交瘤细胞呈现出更好的生长方式，并提高了液化珠粒内的活力和抗体产生。但是，当通过抗压强度研究评估这些珠子时，发现它们在机械上比固态珠子更易碎。为了解决这个问题，我们将非自体细胞（BHK成纤维细胞和C2C12成肌细胞）包埋在固体藻酸盐-琼脂糖珠中，并观察到它们的生长特性得到了改善，并且其存活时间延长了70天（相比之下，观察到的15天）。杂交瘤细胞。

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