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首页> 外文期刊>Biochemical Systematics and Ecology >Analysis of the genetic diversity of garlic (Allium sativum L.) by simple sequence repeat and inter simple sequence repeat analysis and agro-morphological traits
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Analysis of the genetic diversity of garlic (Allium sativum L.) by simple sequence repeat and inter simple sequence repeat analysis and agro-morphological traits

机译：通过简单重复序列和内部简单重复序列分析及农业形态性状分析大蒜（Allium sativum L.）的遗传多样性

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摘要

The genetic diversity of 39 garlic accessions was investigated using eight simple sequence repeat (SSR) primer combinations and 17 inter-simple sequence repeat (ISSR) primer combinations. A total of 109 polymorphic loci were detected among these accessions, with an average of 4.63 polymorphic loci per SSR primer combination and 4.29 polymorphic loci per ISSR primer combination. The mean effective number of alleles, the mean Nei's genetic diversity, and the mean Shannon's information index for SSR were 1.4799, 0.2870, and 0.4378, respectively; and those for ISSR were 1.4847, 0.2898 and 0.4415, respectively. Cluster analysis, using the unweighted pair-group method with arithmetic averages (UPGMA) based on the allele frequency data, classified the accessions into three groups. The results of principal component analysis (PCA) were consistent with those of the cluster analysis. PCA showed that each of these three groups exhibited significant variation in agro-morphological traits. These findings suggest that the eight SSR and 17 ISSR primers identified could define valuable markers for genetic diversity for use by plant breeders
机译：使用8个简单序列重复（SSR）引物组合和17个简单序列间重复（ISSR）引物组合研究了39个大蒜种的遗传多样性。在这些种质中总共检测到109个多态性基因座，每个SSR引物组合平均有4.63个多态性基因座，每个ISSR引物组合平均有4.29个多态性基因座。 SSR的平均等位基因有效数，平均Nei遗传多样性和Shannon信息指数平均分别为1.4799、0.2870和0.4378。 ISSR分别为1.4847、0.2898和0.4415。聚类分析使用等位基因频率数据基于算术平均值的非加权成对组方法（UPGMA），将种质分为三组。主成分分析（PCA）的结果与聚类分析的结果一致。 PCA显示这三组中的每组在农业形态性状上均表现出显着变化。这些发现表明，鉴定出的八种SSR和17种ISSR引物可以为植物育种者使用的遗传多样性定义有价值的标记

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