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首页> 外文期刊>Experimental and therapeutic medicine >Expression and significance of 90K/Mac-2BP in prostate cancer
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Expression and significance of 90K/Mac-2BP in prostate cancer

机译：90K / Mac-2BP在前列腺癌中的表达及意义

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The aim of this study was to detect the differences in 90K/Mac-2BP expression in prostate cancer, benign prostatic hyperplasia and normal prostate tissues, as well as to study the significance of 90K/Mac-2BP in the early diagnosis and prognosis of prostate cancer. Comparative proteomic technologies were used in the present study. Total protein from 10 cases of prostate cancer, benign prostatic hyperplasia and normal prostate tissue was extracted and separated by two-dimensional electrophoresis (2-DE). Proteins expressed differentially by more than 2-fold were selected for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MS) and biological information analysis. The 2-DE patterns of the proteins from the normal prostate, benign prostatic hyperplasia and prostate cancer tissues were successfully identified. The average numbers of protein spots were 3,066, 3,289 and 2,986, respectively. There were 31 spots with a difference of more than 2-fold. A total of 18 proteins were identified by MS and database searches. Of these 18 proteins, the most significant differential expression was that of 90K/Mac-2BP. Functional analysis demonstrated that 90K/Mac-2BP (Mac-2 binding protein) overexpression is correlated with the occurrence, proliferation, differentiation and metastasis of cancer cells. The proteomic approach used in the present study was effective and is feasible for identifying differentially expressed proteins in prostate cancer, benign prostatic hyperplasia and normal prostate tissues. 90K/Mac-2BP may be important for the early diagnosis and prognosis of prostate cancer and may also be associated with the molecular mechanisms of prostate cancer development.
机译：这项研究的目的是检测前列腺癌，良性前列腺增生和正常前列腺组织中90K / Mac-2BP表达的差异，以及研究90K / Mac-2BP在前列腺癌的早期诊断和预后中的意义。癌症。比较蛋白质组学技术被用于本研究中。提取10例前列腺癌，良性前列腺增生和正常前列腺组织中的总蛋白，并通过二维电泳（2-DE）进行分离。选择差异表达超过2倍的蛋白质进行基质辅助激光解吸/电离飞行时间质谱（MS）和生物学信息分析。已成功鉴定出来自正常前列腺，良性前列腺增生和前列腺癌组织的蛋白质的2-DE模式。蛋白质斑点的平均数量分别为3,066、3,289和2,986。有31个斑点，差异超过2倍。通过质谱和数据库搜索共鉴定出18种蛋白质。在这18种蛋白质中，最显着的差异表达是90K / Mac-2BP。功能分析表明90K / Mac-2BP（Mac-2结合蛋白）的过表达与癌细胞的发生，增殖，分化和转移有关。在本研究中使用的蛋白质组学方法是有效的，并且对于鉴定前列腺癌，良性前列腺增生和正常前列腺组织中差异表达的蛋白质是可行的。 90K / Mac-2BP对前列腺癌的早期诊断和预后可能很重要，也可能与前列腺癌发展的分子机制有关。

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