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首页> 外文期刊>European Journal of Obstetrics, Gynecology and Reproductive Biology: An International Journal >Gene expression and protein localization of TLR-1, -2, -4 and -6 in amniochorion membranes of pregnancies complicated by histologic chorioamnionitis
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Gene expression and protein localization of TLR-1, -2, -4 and -6 in amniochorion membranes of pregnancies complicated by histologic chorioamnionitis

机译：妊娠合并组织学绒毛膜羊膜炎的羊膜结构中TLR-1，-2，-4和-6的基因表达和蛋白定位

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Objectives To determine whether histologic chorioamnionitis is associated with changes in gene expression of TLR-1, -2, -4 and -6, and to describe the localization of these receptors in fetal membranes. Study design A total of 135 amniochorion membranes with or without histologic chorioamnionitis from preterm or term deliveries were included. Fragments of membranes were submitted to total RNA extraction. RNA was reverse transcribed and the quantification of TLRs expression measured by real time PCR. Results All amniochorion membranes expressed TLR-1 and TLR-4, whereas 99.1% of membranes expressed TLR-2 and 77.4% expressed TLR-6. TLR-1 and TLR-2 expressions were significantly higher in membranes with histologic chorioamnionitis as compared to membranes without chorioamnionitis in preterm pregnancies (p = 0.003 and p < 0.001, respectively). Among the membranes of term pregnancies there were no differences in the expressions of such receptors regardless of inflammatory status. Regarding TLR-4 and TLR-6 expression, there was no difference among membranes with or without histologic chorioamnionitis, regardless gestational age at delivery. TLR-1, TLR-2, TLR-4 and TLR-6 expressions were observed in amniotic epithelial, chorionic and decidual cells. Conclusion Amniochorion membranes express TLR-1, TLR-2, TLR-4 and TLR-6 and increased expression of TLR-1 and TLR-2 is related to the presence of histologic chorioamnionitis in preterm pregnancies. This study provides further evidence that amniochorion membranes act as a mechanical barrier to microorganisms and as components of the innate immune system.
机译：目的确定组织学绒毛膜羊膜炎是否与TLR-1，-2，-4和-6基因表达的变化有关，并描述这些受体在胎膜中的定位。研究设计包括总共135个早产或足月分娩的有或没有组织学绒毛膜羊膜炎的羊膜膜。膜的片段用于总RNA提取。 RNA被逆转录并且通过实时PCR测量TLR表达的定量。结果所有羊膜蛋白膜均表达TLR-1和TLR-4，而99.1％的膜表达TLR-2和77.4％的膜表达TLR-6。在早孕中，组织学绒毛膜羊膜炎的膜中的TLR-1和TLR-2表达明显高于无绒毛膜羊膜炎的膜（分别为p = 0.003和p <0.001）。在足月妊娠的胎膜之间，不管炎症状态如何，这种受体的表达都没有差异。关于TLR-4和TLR-6的表达，无论是否分娩的胎龄，在有或没有组织学绒毛膜羊膜炎的膜之间没有差异。在羊膜上皮，绒毛膜和蜕膜细胞中观察到TLR-1，TLR-2，TLR-4和TLR-6的表达。结论羊膜蛋白表达TLR-1，TLR-2，TLR-4和TLR-6，TLR-1和TLR-2表达增加与早孕组织性绒膜羊膜炎的存在有关。这项研究提供了进一步的证据，羊膜蛋白膜可作为对微生物的机械屏障和先天免疫系统的组成部分。

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