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首页> 外文期刊>Expert opinion on biological therapy >Development of 2A peptide-based strategies in the design of multicistronic vectors.
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Development of 2A peptide-based strategies in the design of multicistronic vectors.

机译：在多顺反子载体设计中开发基于2A肽的策略。

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摘要

As science progresses in its understanding of diseases and their treatment, advances have been made in the biotechnology used in disease therapy. Most gene therapy approaches utilise viral vectors to deliver genes of interest. However, multiple proteins are often involved in disease processes and there is often a need to efficiently deliver more than one gene. Researchers have employed several strategies to accomplish this goal. When designing vectors to express multiple genes, there are several factors that need to be taken into account, including cell type, the activity of the protein of interest and subcellular protein localisation. In most cases, it is ideal for each protein to be expressed at comparable levels, a leading issue with traditional strategies for multigene expression. This review describes some of the techniques that have been used to express multiple genes, and will focus on the use of 2A peptides or 2A peptide-like sequences in the design of multicistronic vectors that may alleviate some of these issues.
机译：随着科学对疾病及其治疗方法的理解的进步，用于疾病治疗的生物技术也取得了进步。大多数基因治疗方法都利用病毒载体来传递目标基因。然而，多种蛋白质通常参与疾病过程，并且经常需要有效地递送多个基因。研究人员采用了几种策略来实现这一目标。当设计表达多个基因的载体时，需要考虑几个因素，包括细胞类型，目的蛋白的活性和亚细胞蛋白的定位。在大多数情况下，理想的是使每种蛋白质均以可比较的水平表达，这是传统的多基因表达策略的首要问题。这篇综述描述了已用于表达多个基因的一些技术，并将着重于在设计多顺反子载体中使用2A肽或2A肽样序列，以减轻其中的一些问题。

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