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首页> 外文期刊>Genetic Resources and Crop Evolution >Marker-assisted acquisition and core collection formation: a case study in barley using AFLPs and pedigree data.
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Marker-assisted acquisition and core collection formation: a case study in barley using AFLPs and pedigree data.

机译：标记辅助收购和核心收藏形成：使用AFLP和谱系数据的大麦案例研究。

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摘要

A problem that often occurs in deciding which germplasm should be acquired to expand the diversity of a plant genetic resources collection, and which accessions should be included in a core collection, is the lack of proper data. The usefulness of an AFLP-based protocol to assist in acquisition decisions and in core collection formation was examined by using 52 barley cultivars. For validation purposes, pedigree data of the cultivars were used to calculate the 'effective number of origin lines' (no1), a parameter introduced in earlier research that was defined as the number of alleles per locus, not identical by descent, in a set of lines. Two AFLP primer combinations were able to distinguish all 52 cultivars from each other, and to discriminate between spring and winter crop types. Using the year of origin of the cultivars, the historical development of no1 showed a stepwise pattern, indicating the periodical release of genetically similar cultivars, alternated by the incorporation of new material. Comparison of AFLP data between cultivars and both their parents was possible in five cases. These comparisons revealed a high likelihood that the correct parents were involved but a rather skewed contribution of parents to offspring, suggesting that backcrossing had been applied. Treating the 25 cultivars that were released before 1980 and played an important role in barley cultivation as a basic collection, and the 27 more recent cultivars as potential candidates for acquisition, no1 values generated by a marker-based approach largely followed those using a random approach. Given this poor performance, a marker-based protocol to assist in acquisition decisions was not considered useful for the analysed material. If the 52 cultivars were considered to be the collection from which a core collection had to be selected, the marker-based selection showed much better results compared to a random selection. About half of the total number of origin lines could be captured with a quarter of the collection, indicating the potential utility of AFLPs in core collection formation..
机译：缺乏适当的数据往往是一个问题，即决定应获取哪种种质资源来扩大植物遗传资源集合的多样性，以及哪些种应包括在核心集合中。通过使用52个大麦品种，研究了基于AFLP的方案有助于进行收购决策和核心品系形成的有用性。为了验证目的，使用品种的系谱数据来计算“有效原代数”（no1），这是早先研究中引入的参数，定义为每个基因座中等位基因的数量，在下降时并不相同线。两种AFLP引物组合能够区分所有52个品种，并区分春季和冬季农作物类型。使用品种的起源年份，no1的历史发展呈逐步模式，这表明遗传相似的品种会定期释放，并通过掺入新材料而交替出现。在五种情况下，有可能对两个品种及其父母之间的AFLP数据进行比较。这些比较表明，正确父母的参与可能性很高，但父母对后代的贡献偏颇，这表明已经进行了回交。将1980年之前发布的在大麦栽培中发挥重要作用的25个品种作为基本收藏，将最近的27个品种作为可能的收购对象，基于标记的方法产生的no1值很大程度上跟随使用随机方法的那些。鉴于这种较差的性能，认为基于标记的方案来帮助进行采集决策对于分析的材料不起作用。如果认为52个品种是必须从中选择核心品种的品种，则与随机选择相比，基于标记的选择显示出更好的结果。大约有四分之一的采集可以捕获到原始线总数的一半，这表明AFLP在核心采集形成中的潜在效用。

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