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首页> 外文期刊>Genetic Resources and Crop Evolution >Induction and assessment of morpho-biochemical mutants in Artemisia pallens Bess.
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Induction and assessment of morpho-biochemical mutants in Artemisia pallens Bess.

机译：青蒿Bess中形态生化突变体的诱导和评估。

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Artemisia pallens Bess. is a low volume and high value essential oil plant used in perfumery, cosmetic and flavouring industries. On account of the failure of conventional procedures to induce variability in species, mutation techniques have been tried in our experiments. Dry and viable seeds (moisture content 8%) of homozygous pure breeding lines were subjected to 150-500 Gy doses of gamma rays and 0.01-0.1% ethyl methane sulphonate (EMS) for 8 h. Desirable qualitative mutants were recovered from segregating M2 generation (4,283 plants scored) raised as single plant progenies. The spectrum of morphological mutants included late and early flowering types; bushy and high yielding types; tall and more capitula-producing types and high oil and high davanone yielding types. These were raised through M3 families to evaluate stability and transmission of mutant characters. As such out of 15 different types selected in M2, only 11 types bred true to their characteristic variability. Based on their performance, the mutants were characterised depending upon their distinguishing features. Davanone, the main component of oil showed the maximum increase (64.22% against 54.64% in control) in mutant 'S-5' recovered from exposure with 250 Gy gamma -rays. Mutant 'E-6' was economically most viable having increased oil biosynthesis (0.36% against 0.22% in control) and hence yields higher oil per unit area than the parental control (isolated from 0.05% EMS treatment).
机译：蒿蒿贝丝。是用于香水，化妆品和调味品行业的小批量，高价值的精油植物。由于常规程序无法诱导物种变异，因此在我们的实验中尝试了突变技术。纯合纯种系的干燥且有活力的种子（水分含量为8％）要接受150-500 Gy剂量的伽马射线和0.01-0.1％的甲烷磺酸乙酯（EMS）处理8小时。从分离的M2世代（得分4,283株植物）作为单株植物后代中回收了所需的定性突变体。形态突变体的谱图包括晚期和早期开花类型。浓密和高产类型高和更多的capitula生产类型和高油和高达瓦农产量类型。这些是通过M3家族提出的，以评估突变体字符的稳定性和传播。因此，在M2中选择的15种不同类型中，只有11种真正因其特性变异而繁殖。基于它们的性能，根据其区别特征对突变体进行表征。达万隆是石油的主要成分，在暴露于250 Gyγ射线后恢复的突变体“ S-5”中显示出最大的增加（64.22％，对照中为54.64％）。突变体“ E-6”在经济上是最可行的，具有增加的油生物合成（0.36％对对照中的0.22％），因此比亲本对照（从0.05％EMS处理中分离）每单位面积产生的油更高。

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