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首页> 外文期刊>Genome >Fine mapping of the yellow seed locus in Brassica juncea L.
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Fine mapping of the yellow seed locus in Brassica juncea L.

机译：甘蓝型油菜黄色种子基因座的精细定位

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The yellow mustard plant in Northern Shaanxi is a precious germplasm, and the yellow seed trait is controlled by a single recessive gene. In this report, amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) techniques were used to identify markers linked to the brown seed locus in an F_2 population consisting of 1258 plants. After - screening 256 AFLP primer combinations and 456 pairs of SSR primers, we found 14 AFLP and 2 SSR markers that were closely linked to the brown seed locus. Among these markers, the SSR marker CB 1022 showed codominant inheritance. By integrating markers previously found to be linked to the brown seed locus into the genetic map of the F_2 population, 23 markers were linked to the brown seed locus. The two closest markers, EA02MC08 and PO3MC08, were located on either side of the brown seed locus at a distance of 0.3 and 0.5 cM, respectively. To use the markers for the breeding of yellow-seeded mustard plants, two AFLP markers (EA06MC 11 and EA08MC 13) were converted into sequence-characterized amplified region (SCAR) markers, SC1 and SC2, with the latter as the codominant marker. The two SSR markers were subsequently mapped to the A9/N9 linkage group of Brassica napus L. by comparing common SSR markers with the published -genetic map of B. napus. A BLAST analysis indicated that the sequences of seven markers showed good colinearity with those of Arabidopsis chromosome 3 and that the homolog of the brown seed locus might exist between At3g14120 and At3g29615 on this same chromosome. To develop closer markers, we could make use of the sequence information of this region to design primers for future studies. Regardless, the close markers obtained in the present study will lay a solid foundation for cloning the yellow seed gene using a map-based cloning strategy.
机译：陕北黄芥菜是一种珍贵的种质，其黄色种子性状由一个隐性基因控制。在本报告中，扩增片段长度多态性（AFLP）和简单序列重复（SSR）技术用于鉴定与1258株植物组成的F_2群体中的棕色种子基因座相关的标记。在筛选了256种AFLP引物组合和456对SSR引物后，我们发现14个AFLP和2个SSR标记与棕色种子基因座紧密相关。在这些标记中，SSR标记CB 1022显示出显性遗传。通过将先前发现与棕色种子基因座相关的标记整合到F_2种群的遗传图谱中，将23个标记与棕色种子基因座相关。两种最接近的标记EA02MC08和PO3MC08位于棕色种子基因座的两侧，分别相距0.3和0.5 cM。为了将标记用于黄种芥菜植物的育种，将两个AFLP标记（EA06MC 11和EA08MC 13）转换为序列特征扩增区（SCAR）标记SC1和SC2，后者为共性标记。随后通过将常见的SSR标记与已发表的甘蓝型油菜遗传图谱进行比较，将这两个SSR标记定位到甘蓝型油菜的A9 / N9连锁群。 BLAST分析表明，七个标记的序列与拟南芥第3号染色体具有良好的共线性，并且棕色种子基因座的同源物可能存在于同一染色体的At3g14120和At3g29615之间。要开发更接近的标记，我们可以利用该区域的序列信息来设计引物，以备将来研究。无论如何，本研究中获得的紧密标记将为使用基于图谱的克隆策略克隆黄色种子基因奠定坚实的基础。

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