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A survey of Penstemon's genome size

机译：Penstemon基因组大小的调查

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摘要

Penstemon is the largest genus in North America with more than 270 reported species. However, little is known about its genome size. This information may be useful in developing hybrids for landscape use and for gaining insight into its current taxonomy. Using flow cytometry, we estimated the genome size of approximately 40% of the genus (115 accessions from 105 different species). Genome sizes for both reported and probable diploids range from P. dissectus 2C = 0.94 pg (1C = 462 Mbp) to P. pachyphyllus var. mucronatus 2C = 1.88 pg (1C = 919 Mbp), and the polyploids range from P. attenuatus var. attenuatus 2C = 2.35 pg (1C = 1148 Mbp) to P. digitalis 2C = 6.45 pg (1C = 3152 Mbp). Chromosome counts were done for ten previously published and four previously unreported Penstemon species (P. dissectus, P. navajoa, P. caespitosus var. desertipicti, and P. ramaleyi). These counts were compiled with all previously published chromosome data and compared with the flow cytometry results. Ploidy within this study ranged from diploid to dodecaploid. These data were compared and contrasted with the current taxonomy of Penstemon and previously published internal transcribed spacer and chloroplast DNA phylogenetic work. Based on genome size and previous studies, reassigning P. montanus to the subgenus Penstemon and P. personatus to the subgenus Dasanthera, would better reflect the phylogeny of the genus. Furthermore, our data concur with previous studies suggesting that the subgenus Habroanthus be included in the subgenus Penstemon. The DNA content of subgenus Penstemon exhibits high plasticity and spans a sixfold increase from the smallest to the largest genome (P. linarioides subsp. sileri and P. digitalis, respectively). Our study found flow cytometry to be useful in species identification and verification.
机译：Penstemon是北美最大的属，有超过270种已报道的物种。但是，对其基因组大小知之甚少。此信息可能有助于开发用于景观的混合动力车，并有助于了解其当前的分类法。使用流式细胞仪，我们估计了大约40％属的基因组大小（来自105个不同物种的115个种）。报道的和可能的二倍体的基因组大小范围分别为：假单胞菌2C = 0.94 pg（1C = 462 Mbp）到野假单胞菌。 mucronatus 2C = 1.88 pg（1C = 919 Mbp），多倍体的范围为P. detectus var。衰减2C = 2.35 pg（1C = 1148 Mbp）到洋地黄2C = 6.45 pg（1C = 3152 Mbp）。对先前发表的十种和未报道过的四种Penstemon物种（P. dissectus，P。navajoa，P.caespitosus var.desertipicti和P. ramaleyi）进行了染色体计数。将这些计数与所有先前发布的染色体数据进行汇总，并与流式细胞仪结果进行比较。这项研究中的倍性从二倍体到十二倍体不等。将这些数据与Penstemon的当前分类法以及先前发表的内部转录间隔子和叶绿体DNA系统发生研究进行了对比和对比。根据基因组大小和先前的研究，将P. montanus重新分配给Penstemon属，将P. personatus重新分配给Dasanthera属，会更好地反映该属的系统发育。此外，我们的数据与先前的研究一致，提示Habroanthus属包括在Penstemon属中。 Penstemon亚属的DNA含量具有很高的可塑性，从最小的基因组到最大的基因组分别增长了六倍（分别为linarioides亚种sileri和P. digitalis）。我们的研究发现流式细胞仪可用于物种鉴定和验证。

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