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首页> 外文期刊>Genome >Development and characterization of 248 novel microsatellite markers in turbot (Scophthalmus maximus).
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Development and characterization of 248 novel microsatellite markers in turbot (Scophthalmus maximus).

机译：大菱t（Scophthalmus maximus）中248种新型微卫星标记的开发和表征。

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The turbot is a flatfish species of great relevance to marine aquaculture in Europe. Only a limited number of microsatellites have been isolated to date in this species. To increase the number of potentially useful mapping markers, we screened simple sequence repeat (SSR)--enriched genomic libraries obtained from several di-, tri-, and tetranucleotide tandem repeat motifs. A total of 248 new polymorphic microsatellites were successfully optimized. The efficiency of the protocol applied (6.4%) was higher than that in other studies of fish that used the same method. Dinucleotide and perfect microsatellites were predominant in this species; the (AC)n motif was the most frequent class of repeat. Polymorphism and structural properties at these loci, together with 30 variable loci previously reported in turbot, were evaluated in 6 wild individuals. The number of alleles per locus ranged from 2 to 10, with an average of 4.046. The microsatellite markers characterized in this study will contribute to the development of the turbot genetic map, which can be used for quantitative trait locus (QTL) identification, marker-assisted selection programs, and other applications to improve its culture.
机译：该比目鱼是与欧洲海水养殖极为相关的比目鱼物种。迄今为止，在该物种中仅分离了有限数量的微卫星。为了增加潜在有用的作图标记的数量，我们筛选了从几个双，三和四核苷酸串联重复基序中获得的，富含简单序列重复（SSR）的基因组文库。总共成功地优化了248个新的多态微卫星。所应用方案的效率（6.4％）高于其他使用相同方法的鱼类研究。双核苷酸和完美的微卫星在该物种中占主导地位。 （AC）n主题是最常见的重复类别。在6个野生个体中评估了这些基因座的多态性和结构特性，以及以前在比目鱼中报道的30个可变基因座。每个基因座的等位基因数量范围为2到10，平均为4.046。这项研究中表征的微卫星标记将有助于大菱turbo遗传图谱的发展，该图谱可用于定量性状位点（QTL）鉴定，标记辅助选择程序以及其他改善其培养的应用。

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