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首页> 外文期刊>Genomics >Genome-tagged mice (GTM): two sets of genome-wide congenic strains.
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Genome-tagged mice (GTM): two sets of genome-wide congenic strains.

机译：基因组标记的小鼠（GTM）：两组全基因组的同系品系。

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An important approach for understanding complex disease risk using the mouse is to map and ultimately identify the genes conferring risk. Genes contributing to complex traits can be mapped to chromosomal regions using genome scans of large mouse crosses. Congenic strains can then be developed to fine-map a trait and to ascertain the magnitude of the genotype effect in a chromosomal region. Congenic strains are constructed by repeated backcrossing to the background strain with selection at each generation for the presence of a donor chromosomal region, a time-consuming process. One approach to accelerate this process is to construct a library of congenic strains encompassing the entire genome of one strain on the background of the other. We have employed marker-assisted breeding to construct two sets of overlapping congenic strains, called genome-tagged mice (GTMs), that span the entire mouse genome. Both congenic GTM sets contain more than 60 mouse strains, each with on average a 23-cM introgressed segment (range 8 to 58 cM). C57BL/6J was utilized as a background strain for both GTM sets with either DBA/2J or CAST/Ei as the donor strain. The background and donor strains are genetically and phenotypically divergent. The genetic basis for the phenotypic strain differences can be rapidly mapped by simply screening the GTM strains. Furthermore, the phenotype differences can be fine-mapped by crossing appropriate congenic mice to the background strain, and complex gene interactions can be investigated using combinations of these congenics. Copyright 2001 Academic Press.
机译：使用鼠标了解复杂疾病风险的一种重要方法是定位并最终确定赋予风险的基因。可以使用大型小鼠杂交的基因组扫描将有助于复杂性状的基因定位到染色体区域。然后可以开发出同系菌株，以细化性状并确定染色体区域中基因型效应的强度。通过重复回交到背景菌株来构建同质菌株，并在每一代选择供体染色体区域的存在，这是一个耗时的过程。一种加速此过程的方法是构建一个同系菌株的文​​库，该库包含一个菌株的另一基因组的整个基因组。我们采用了标记辅助育种来构建两组覆盖整个小鼠基因组的重叠同系品系，称为基因组标记小鼠（GTM）。这两个同基因的GTM集都包含60多个小鼠品系，每个品系平均具有一个23 cM的渗入片段（范围8至58 cM）。 C57BL / 6J用作两个GTM组的背景菌株，DBA / 2J或CAST / Ei作为供体菌株。背景和供体菌株在遗传和表型上是不同的。通过简单地筛选GTM菌株，可以快速定位表型菌株差异的遗传基础。此外，可以通过将合适的同基因小鼠与背景菌株杂交来细化表型差异，并可以使用这些同基因的组合研究复杂的基因相互作用。版权所有2001，学术出版社。

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