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首页> 外文期刊>Genomics >Fractionation of chromosome 15 with an affinity-based approach using magnetic beads.
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Fractionation of chromosome 15 with an affinity-based approach using magnetic beads.

机译：使用基于磁珠的基于亲和力的方法分离15号染色体。

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摘要

The two main shortcomings of the state-of-the-art method of sorting chromosomes, specificity and the efficiency of fractionating a significant amount of chromosomes, are addressed by this work in the design of a massively parallel approach using magnetic beads binding to a chromosome-specific DNA probe. In an attempt to isolate human chromosome 15 from a lymphoblastoid cell line, a chromosome 15 centromere-specific DNA probe with a fluorescent tag attached was reacted with the chromosomes. Magnetic beads bound to anti-FITC antibody were reacted with the labeled pool of chromosomes and separated by exposure to a magnetic field. The specificity of the fractionated pool was verified by performing fluorescence in situ hybridization on the isolated pool. The chromosome of interest could be enriched to about 75% within a maximum of 3-4 days, regardless of the amount of material.
机译：这项工作在使用磁珠与染色体结合的大规模平行方法设计中解决了目前最先进的染色体分选方法的两个主要缺点，特异性和大量染色体分馏的效率。特异性DNA探针。为了从淋巴母细胞系中分离出人15号染色体，使带有荧光标签的15号染色体着丝粒特异性DNA探针与该染色体反应。使与抗FITC抗体结合的磁珠与标记的染色体库发生反应，并通过暴露于磁场进行分离。通过对分离的池进行荧光原位杂交来验证分馏池的特异性。无论材料量如何，目标染色体最多可在3-4天之内富集到约75％。

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