...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Genomics >Complex organization and structure of sense and antisense transcripts expressed from the DIO3 gene imprinted locus.
【24h】

Complex organization and structure of sense and antisense transcripts expressed from the DIO3 gene imprinted locus.

机译：从DIO3基因印迹的位点表达的有义和反义转录物的复杂组织和结构。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The human DIO3 gene and its mouse homolog, Dio3, map to chromosomes 14q32 and 12F1, respectively, and code for the type 3 deiodinase, an enzyme that inactivates thyroid hormones and is highly expressed during pregnancy and development. Mouse Dio3 is imprinted and preferentially expressed from the paternal allele in the fetus. We analyzed the human DIO3 genomic region and identified a gene (DIO3OS) that is transcribed in the antisense orientation. Multiple DIO3OS transcripts are expressed in most tissues. The structure of several DIO3OS cDNAs obtained by RT-PCR-based techniques reveals the occurrence of numerous splice variants. The exon-intron structures of DIO3OS are similar in mouse and human, but the homology of the exonic sequence is very low, except for the first exon, and no conserved open reading frame is present. We also detected DIO3 transcripts containing additional 5' untranslated sequence and a potential alternative upstream promoter for mouse Dio3. Exonic sequence of a Dio3os cDNA overlapswith the Dio3 promoter and strong promoter activity in the antisense orientation is detected in a genomic fragment located 3' of mouse and human DIO3 but not in the DIO3 promoter region. These results suggest that the DIO3 gene may lie within the structure of the antisense gene, a complex arrangement often observed in imprinted loci.
机译：人类DIO3基因及其小鼠同源物Dio3分别映射到染色体14q32和12F1，并编码3型脱碘酶，该酶使甲状腺激素失活，并在妊娠和发育过程中高度表达。小鼠Dio3的印迹和优先从胎儿的父本等位基因表达。我们分析了人类DIO3基因组区域，并确定了以反义方向转录的基因（DIO3OS）。在大多数组织中表达多种DIO3OS转录本。通过基于RT-PCR的技术获得的几个DIO3OS cDNA的结构揭示了许多剪接变体的出现。 DIO3OS的外显子-内含子结构在小鼠和人类中相似，但是除第一个外显子外，外显子序列的同源性非常低，并且不存在保守的开放阅读框。我们还检测到含有其他5'非翻译序列和小鼠Dio3潜在替代上游启动子的DIO3转录本。 Dio3os cDNA的外显子序列与Dio3启动子重叠，并且在位于小鼠和人DIO3 3'的基因组片段中（但不在DIO3启动子区域中）检测到反义方向的强启动子活性。这些结果表明，DIO3基因可能位于反义基因的结构内，这是在印迹基因座中经常观察到的复杂排列。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号