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首页> 外文期刊>Genomics >Genome-wide identification of genes that play a role in boron stress response in yeast.
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Genome-wide identification of genes that play a role in boron stress response in yeast.

机译：全基因组范围内鉴定在酵母中的硼胁迫反应中起作用的基因。

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Boron is an essential micronutrient for plants and it is either necessary or beneficial for animals. Studies identified only few genes related to boron metabolism thus far and details of how boron is imported into cells and used in cell metabolism are largely unknown. In order to identify genes that play roles in boron metabolism, we screened the entire set of yeast haploid deletion mutants and identified 6 mutants that were resistant to toxic levels of boron, and 21 mutants that were highly sensitive to boron treatment. Furthermore, we performed a proteomic approach to identify additional proteins that are significantly up-regulated by boron treatment. Our results revealed many genes and pathways related to boron stress response and suggest a possible link between boron toxicity and translational control.
机译：硼是植物必需的微量营养素，对动物来说是必需的或有益的。到目前为止，研究仅发现了与硼代谢有关的基因，而且硼如何导入细胞并用于细胞代谢的细节尚不清楚。为了鉴定在硼代谢中起作用的基因，我们筛选了整套酵母单倍体缺失突变体，并鉴定了6种对硼毒性水平具有抗性的突变体和21种对硼处理高度敏感的突变体。此外，我们进行了蛋白质组学研究，以鉴定通过硼处理显着上调的其他蛋白质。我们的结果揭示了许多与硼胁迫反应相关的基因和途径，并暗示了硼毒性与翻译控制之间的可能联系。

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