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首页> 外文期刊>Genomics >Human carbonic anhydrase XIV (CA14): cDNA cloning, mRNA expression, and mapping to chromosome 1.
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Human carbonic anhydrase XIV (CA14): cDNA cloning, mRNA expression, and mapping to chromosome 1.

机译：人碳酸酐酶XIV（CA14）：cDNA克隆，mRNA表达并定位于1号染色体。

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摘要

A full-length cDNA clone of a human carbonic anhydrase XIV (HGMW-approved gene symbol CA14) was obtained and sequenced. The cDNA sequence was 1757 bp long and was predicted to encode a 337-amino-acid polypeptide with a molecular mass of 37.6 kDa. The deduced amino acid sequence of CA XIV showed an overall similarity of 29-46% to other active CA isozymes. The highest percentage similarity was with a transmembrane CA isoform, CA XII. As observed for CA XII, CA XIV has hydrophobic segments at both termini of the deduced protein for a putative signal sequence and a transmembrane domain. CA XIV showed low activity and was sensitive to acetazolamide, but not to sulfonamide. Northern blot analysis demonstrated an approximately 1.7-kb transcript in the adult human heart, brain, liver, and skeletal muscle. RNA dot-blot analysis for CA XIV mRNA expression showed a strong signal in all parts of the human brain and a weaker signal in the colon, small intestine, urinary bladder, and kidney. RT-PCR analysis showed an intense signal in the liver and spinal cord and a faint signal in the kidney. No CA XIV mRNA was seen in the salivary gland and pancreas. In contrast, CA XII mRNA was expressed in the kidney, salivary gland, and pancreas, but not in the liver or spinal cord. The CA XIV gene was localized to human chromosome 1q21. These findings indicate genetically distinct but closely related isoforms of human transmembrane CAs, CA XII and CA XIV, which have different patterns of tissue-specific expression. Copyright 1999 Academic Press.
机译：获得了人类碳酸酐酶XIV的全长cDNA克隆（HGMW批准的基因符号CA14）并进行了测序。 cDNA序列长1757 bp，并被预测为编码具有37.6 kDa分子量的337个氨基酸的多肽。 CA XIV推导的氨基酸序列显示出与其他活性CA同工酶的总体相似性为29-46％。跨膜CA同工型CA XII的相似性百分比最高。如对于CA XII所观察到的，CA XIV在推定的蛋白质序列的推定信号序列和跨膜结构域的末端均具有疏水性片段。 CA XIV活性低，对乙酰唑胺敏感，但对磺酰胺不敏感。 Northern印迹分析表明，成年人的心脏，大脑，肝脏和骨骼肌中的转录本约为1.7kb。 CA XIV mRNA表达的RNA点印迹分析在人脑的所有部位均显示强信号，而在结肠，小肠，膀胱和肾脏中的信号较弱。 RT-PCR分析显示在肝脏和脊髓中有强烈的信号，在肾脏中有微弱的信号。在唾液腺和胰腺中未观察到CA XIV mRNA。相反，CA XII mRNA在肾脏，唾液腺和胰腺中表达，但在肝或脊髓中不表达。 CA XIV基因位于人类1q21号染色体上。这些发现表明人类跨膜CA，CA XII和CA XIV的遗传学上独特但密切相关的同工型，具有不同的组织特异性表达模式。版权所有1999 Academic Press。

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