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首页> 外文期刊>Genomics >Negative energy balance affects imprint stability in oocytes recovered from postpartum dairy cows
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Negative energy balance affects imprint stability in oocytes recovered from postpartum dairy cows

机译：负能量平衡影响从产后奶牛中回收的卵母细胞的印迹稳定性

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Ovarian follicle development in post-partum, high-producing dairy cows, occurs in a compromised endogenous metabolic environment (referred to as negative energy balance, NEB). Key events that occur during oocyte/follicle growth, such as the vital process of genomic imprinting, may be detrimentally affected by this altered ovarian environment. Imprinting is crucial for placental function and regulation of fetal growth, therefore failure to establish and maintain imprints during oocyte growth may contribute to early embryonic loss. Using ovum pick-up (OPU), oocytes and follicular fluid samples were recovered from cows between days 20 and 115 post-calving, encompassing the NEB period. In a complimentary study, cumulus oocyte complexes were in vitro matured under high non-esterified fatty acid (NEFA) concentrations and in the presence of the methyl-donor S-adenosylmethionine (SAM). Pyrosequencing revealed the loss of methylation at several imprinted loci in the OPU derived oocytes. The loss of DNA methylation was observed at the PLAGL1 locus in oocytes, following in vitro maturation (IVM) in the presence of elevated NEFAs and SAM. Finally, metabolomic analysis of postpartum follicular fluid samples revealed significant differences in several branched chain amino acids, with fatty acid profiles beating similarities to those characteristic of lactating dairy cows. These results provide the first evidence that (1) the postpartum ovarian environment may affect maternal imprint acquisition and (2) elevated NEFAs during IVM can lead to the loss of imprinted gene methylation in bovine oocytes. (C) 2014 Elsevier Inc. All rights reserved.
机译：产后高产奶牛的卵巢卵泡发育发生在受损的内源性代谢环境中（称为负能量平衡，NEB）。这种改变的卵巢环境可能会对卵母细胞/卵泡生长过程中发生的关键事件（如基因组印迹的重要过程）产生不利影响。印记对胎盘功能和胎儿生长的调节至关重要，因此在卵母细胞生长过程中未能建立和维持印记可能会导致早期胚胎丢失。使用卵子采集（OPU），在产犊后20至115天（包括NEB期间）从母牛身上回收卵母细胞和卵泡液样品。在一项补充研究中，卵丘卵母细胞复合物在高非酯化脂肪酸（NEFA）浓度和甲基供体S-腺苷甲硫氨酸（SAM）存在下体外成熟。焦磷酸测序揭示了OPU衍生的卵母细胞中几个印迹基因座的甲基化损失。在NEFA和SAM升高的条件下进行体外成熟（IVM）后，在卵母细胞的PLAGL1位点观察到DNA甲基化的丧失。最后，产后卵泡液样品的代谢组学分析显示，几种支链氨基酸存在显着差异，脂肪酸谱与泌乳奶牛的特征相似。这些结果提供了第一个证据，（1）产后卵巢环境可能会影响母体印记获得，（2）IVM期间NEFA升高会导致牛卵母细胞中印记基因甲基化的丧失。 （C）2014 Elsevier Inc.保留所有权利。

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