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Correct identification of genes from serial analysis of gene expression tag sequences.

机译:从基因表达标签序列的序列分析正确鉴定基因。

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SAGE (serial analysis of gene expression) is a remarkable technique for genome-wide analysis of gene expression. It is crucial to understand the extent to which SAGE can accurately indicate a gene or expressed sequence tag (EST) with a single tag. We analyzed the effect of the size of SAGE tag on gene identification. Our observation indicates that SAGE tags are in general not long enough to achieve the degree of uniqueness of identification originally envisaged. Our observations also indicate that the limitation of using SAGE tag to identify a gene can be overcome by converting SAGE tags into longer 3' EST sequences with the generation of longer cDNA fragments from SAGE tages for gene identification (GLGI) method. (c)2002 Elsevier Science (USA).
机译:SAGE(基因表达的序列分析)是一项用于基因组范围内基因表达分析的出色技术。了解SAGE可以在多大程度上准确指示具有单个标签的基因或表达序列标签(EST)至关重要。我们分析了SAGE标签大小对基因鉴定的影响。我们的观察表明,SAGE标签通常不够长,无法达到最初设想的唯一识别程度。我们的观察结果还表明,可以通过将SAGE标签转换为更长的3'EST序列,并利用SAGE标签为基因识别(GLGI)生成更长的cDNA片段,来克服使用SAGE标签识别基因的局限性。 (c)2002 Elsevier Science(美国)。

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