Construction and characterization of subtractive stage-specific expressed sequence tag (EST) libraries of the pinewood nematode Bursaphelenchus xylophilus.

Kang JS; Lee H; Moon IS

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Construction and characterization of subtractive stage-specific expressed sequence tag (EST) libraries of the pinewood nematode Bursaphelenchus xylophilus.

机译：松材线虫Bursaphelenchus xylophilus的减法阶段特异性表达序列标签（EST）文库的构建和表征。

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To establish expressed sequence tag databases of the two life stages (the dispersal and propagative stages) of pinewood nematode Bursaphelenchus xylophilus, subtractive EST libraries that were specific to the dispersal 4th larval stage (D4S) and the pine-grown propagative mixed (PGPS) stage were constructed by suppressed subtractive hybridization, and annotated by BLASTx and Gene Ontology (GO). A total of 1112 (57.7%) contigs from the D4S-cDNA library and 1215 (46.7%) contigs from the PGPS-specific cDNA libraries had matched BLASTx hits (E

机译：要建立松材线虫Bursaphelenchus xylophilus的两个生命阶段（分散和繁殖阶段）的表达序列标签数据库，应针对散布的第四幼虫阶段（D4S）和松树生长的繁殖混合（PGPS）阶段消减EST文库通过抑制性消减杂交构建基因，并通过BLASTx和基因本体论（GO）进行注释。来自D4S-cDNA文库的总共1112个（57.7％）重叠群和来自PGPS特异性cDNA文库的1215个（46.7％）重叠群具有匹配的BLASTx匹配（E

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《Genomics》 |2009年第1期|共8页
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Kang JS; Lee H; Moon IS;
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正文语种 eng
中图分类遗传学;
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1. Construction and characterization of subtractive stage-specific expressed sequence tag (EST) libraries of the pinewood nematode Bursaphelenchus xylophilus. [J] . Kang JS, Lee H, Moon IS Genomics . 2009,第1期

机译：松材线虫Bursaphelenchus xylophilus的减法阶段特异性表达序列标签（EST）文库的构建和表征。
2. Isolation and characterization of expressed sequence tags (ESTs) from subtracted cDNA libraries of Pennisetum glaucum seedlings [J] . Rabi N. Mishra, Palakolanu Sudhakar Reddy, Suresh Nair, Plant Molecular Biology . 2007,第6期

机译：狼尾草幼苗减去cDNA文库表达序列标签（ESTs）的分离与鉴定
3. Isolation and characterization of expressed sequence tags (ESTs) from subtracted cDNA libraries of Pennisetum glaucum seedlings [J] . Mishra RN, Reddy PS, Nair S, Plant Molecular Biology . 2007,第6期

机译：狼尾草幼苗减去cDNA文库表达序列标签（ESTs）的分离与鉴定
4. Molecular identification and pathway analysis of the introduced pinewood nematode Bursaphelenchus xylophilus [C] . K Metge, W Burgermeister Symposium of the British Crop Protection Council . 2005

机译：引进的Pinewood Nematode Bursaphelenchus Xylophilus的分子鉴定和途径分析
5. FOREST DISEASE SURVEY OF DELAWARE: I. SURVEY AND REMOTE SENSING. II. DISTRIBUTION AND POPULATION DYNAMICS OF THE PINEWOOD NEMATODE, BURSAPHELENCHUS XYLOPHILUS. [D] . ADAMS, JAMES CLEVELAND, III. 1982

机译：特拉华州森林疾病调查：I.调查和遥感。二。松材线虫（Burraphelenchus XYLOPHILUS）的分布和种群动态。
6. Construction and characterization of an expressed sequenced tag library for the mosquito vector Armigeres subalbatus [O] . George F Mayhew, Lyric C Bartholomay, Hang-Yen Kou, 2007

机译：蚊媒亚基蚊的表达序列标签文库的构建与表征
7. Construction and characterization of subtractive stage-specific expressed sequence tag (EST) libraries of the pinewood nematode Bursaphelenchus xylophilus [O] . Kang Jae Soon, Lee Hyoungseok, Moon Il Sung, 2009

机译：松材线虫Bursaphelenchus xylophilus减法阶段特异性表达序列标签（EST）文库的构建和表征

Construction and characterization of subtractive stage-specific expressed sequence tag (EST) libraries of the pinewood nematode Bursaphelenchus xylophilus.

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