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STARR-seq - Principles and applications

机译：STARR-seq-原理和应用

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Differential gene expression is the basis for cell type diversity in multicellular organisms and the driving force of development and differentiation. It is achieved by cell type-specific transcriptional enhancers, which are genomic DNA sequences that activate the transcription of their target genes. Their identification and characterization is fundamental to our understanding of gene regulation. Features that are associated with enhancer activity, such as regulatory factor binding or histone modifications can predict the location of enhancers. Nonetheless, enhancer activity can only be assessed by transcriptional reporter assays. Over the past years massively parallel reporter assays have been developed for large scale testing of enhancers. In this review we focus on the principles and applications of STARR-seq, a functional assay that quantifies enhancer strengths in complex candidate libraries and thus allows activity-based enhancer identification in entire genomes. We explain how STARR-seq works, discuss current uses and give an outlook to future applications. (C) 2015 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license.
机译：差异基因表达是多细胞生物中细胞类型多样性的基础，也是发展和分化的动力。它是通过细胞类型特异性转录增强子实现的，后者是激活其靶基因转录的基因组DNA序列。它们的鉴定和表征对于我们对基因调控的理解至关重要。与增强子活性相关的特征，例如调节因子结合或组蛋白修饰，可以预测增强子的位置。尽管如此，增强子活性只能通过转录报告基因分析来评估。在过去的几年中，已经开发了用于增强子的大规模测试的大规模平行报告基因测定。在这篇综述中，我们集中于STARR-seq的原理和应用，STARR-seq是一种功能测定，可以量化复杂候选库中的增强子强度，因此可以在整个基因组中鉴定基于活性的增强子。我们将解释STARR-seq的工作原理，讨论当前用途并展望未来的应用。 （C）2015作者。由Elsevier Inc.发行。这是CC BY-NC-ND许可下的开放获取文章。

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