• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Genes and environment >Spontaneous and Induced gpt and Spi－ Mutant Frequencies in gpt delta Transgenic Rodents
【24h】

Spontaneous and Induced gpt and Spi－ Mutant Frequencies in gpt delta Transgenic Rodents

机译：gpt delta转基因啮齿动物的自发诱导gpt和Spi－突变频率

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Transgenic rodent mutation assays are useful models for investigating the genotoxicity of chemicals in vivo. Transgenic gpt delta mice contain multiple copies of chromosomallyintegrated lambda EG10 phage shuttle vector, which contains reporter genes that allow detection of mutations. This system can identify both point mutations by the gpt assay (6-thioguanine selection) and certain types of deletions using the Spi－ assay. Transgenic gpt delta rats, which have the same lambda EG10 DNA copies as gpt delta mice, have also been developed. The average spontaneous gpt mutant frequency (MF) in both gpt delta mice and rats is approximately 4.5×10－6. In the Spi－ assay, the average spontaneous Spi－ MF is approximately 2.7×10－6 in gpt delta mice, similar to that of gpt delta rats. More than 20 chemicals and irradiations have been analyzed with these systems, and this review summarizes the MFs and treatment conditions. The data demonstrate that these transgenic rodent models are useful for detection and analysis of point mutations and deletions in vivo.
机译：转基因啮齿动物突变试验是用于研究体内化学物质的遗传毒性的有用模型。转基因的gpt delta小鼠包含染色体整合的λEG10噬菌体穿梭载体的多个拷贝，该载体包含允许检测突变的报告基因。该系统既可以通过gpt分析（6-硫鸟嘌呤选择）识别点突变，又可以使用Spi－分析识别某些类型的缺失。还开发了与gpt delta小鼠具有相同λEG10 DNA拷贝的转基因gpt delta大鼠。 gpt三角洲小鼠和大鼠的平均自发性gpt突变频率（MF）约为4.5×10－6。在Spi－分析中，与gptδ大鼠相似，gptδ小鼠的平均自发Spi－ MF约为2.7×10－6。这些系统已经分析了20多种化学物质和辐射，这篇综述总结了MF和治疗条件。数据表明，这些转基因啮齿动物模型可用于体内点突变和缺失的检测和分析。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号