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首页> 外文期刊>Islets >Maintenance of aerobic metabolism increases immunoisolated islet survival.
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Maintenance of aerobic metabolism increases immunoisolated islet survival.

机译：有氧代谢的维持增加了免疫隔离的胰岛的存活。

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The progress of immunoisolation as a treatment for diabetes has been hampered by the diminished long term viability of islets within the immunoisolation device. Chronic hypoxia is greatly responsible for islet cell death within an immunoisolation device and remains an obstacle to the success of this form of islet transplantation. In order to address this problem, isolated rat islets were transfected with a plasmid encoding cytoglobin, an intracellular oxygen binding protein. Untreated or transfected islets were placed in polyacrylonitrile-polyvinychloride hollow fiber and implanted beneath the hepatic capsule in streptozotocin-diabetic rats. Fasting blood glucose was used as an indicator of islet survival and function. Rats receiving fibers containing transfected islets remained normoglycemic through the 60 day trial. Untreated islets failed within two weeks after implantation resulting in elevated blood glucose in the recipient. The fibers were recovered and tested for insulin content. Cytoglobin promoted islet cell survival and insulin synthesis and secretion. The induction of cytoglobin in islets may reduce cell loss from chronic hypoxia and may be a useful method to improve the feasibility of immunoisolation as an islet transplantation modality.
机译：免疫隔离装置中胰岛的长期生存能力降低已阻碍了免疫隔离作为糖尿病治疗的进展。慢性缺氧是免疫隔离装置中胰岛细胞死亡的主要原因，并且仍然是这种形式的胰岛移植成功的障碍。为了解决这个问题，将分离的大鼠胰岛用编码细胞红蛋白（一种细胞内氧结合蛋白）的质粒转染。将未经治疗或转染的胰岛置于聚丙烯腈-聚氯乙烯中空纤维中，并植入链脲佐菌素-糖尿病大鼠肝胶囊下方。空腹血糖被用作胰岛存活和功能的指标。在60天的试验中，接受含有转染胰岛纤维的大鼠的血糖正常。未经治疗的胰岛在植入后两周内失败，导致受体中的血糖升高。回收纤维并测试胰岛素含量。细胞球蛋白促进胰岛细胞存活以及胰岛素合成和分泌。胰岛中细胞红蛋白的诱导可减少慢性缺氧引起的细胞丢失，并且可能是提高免疫分离作为胰岛移植方式的可行性的有用方法。

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