MUTATIONS AT POSITIONS 13 AND/OR 914 IN ESCHERICHIA COLI 16S RIBOSOMAL RNA INTERFERE WITH THE INITIATION OF PROTEIN SYNTHESIS

Pinard R.; Brakiergingras L.; Payant C.

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MUTATIONS AT POSITIONS 13 AND/OR 914 IN ESCHERICHIA COLI 16S RIBOSOMAL RNA INTERFERE WITH THE INITIATION OF PROTEIN SYNTHESIS

机译：大肠埃希氏菌16S核糖体RNA位置13和/或914的突变会干扰蛋白质合成的启动

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Mutations at positions 13 (U-->A) and/or 914 (A-->U) of Escherichia coil 16S rRNA severely affect cell growth and protein synthesis, when expressed in vivo in a vector encoding an rrn operon under control of an inducible promoter, In vitro assays using extension inhibition indicate that the mutations interfere with the formation of the 30S translational initiation complex, which can account for their effect on cell growth. The two mutations destabilize an adjacent pseudoknot helix in which bases 17-19 pair to bases 916-918. This was shown by the increased binding of an oligodeoxyribonucleotide probe complementary to one strand of the pseudoknot helix, and by the increased reactivity to kethoxal of base G917 within this helix. These observations suggest that this pseudoknot helix participates in the formation of the 30S translational initiation complex. [References: 29]

机译：当在编码rrn操纵子的载体中体内表达时，大肠杆菌线圈16S rRNA的13位（U→A）和/或914（A→U）处的突变会严重影响细胞生长和蛋白质合成。诱导型启动子，使用延伸抑制的体外分析表明，突变会干扰30S翻译起始复合物的形成，这可以解释其对细胞生长的影响。这两个突变使附近的假结螺旋不稳定，其中碱基17-19与916-918配对。这通过与假结螺旋的一条链互补的寡脱氧核糖核苷酸探针的结合增加，以及在该螺旋内对碱基G917的对乙二醛的反应性增加而表明。这些观察结果表明该假结螺旋参与了30S翻译起始复合物的形成。 [参考：29]

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《Biochemistry》 |1995年第29期|共6页
作者
Pinard R.; Brakiergingras L.; Payant C.;
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正文语种 eng
中图分类生物化学;
关键词
30s subunit; Conformational switch; Primer extension; Messenger-rna; Translation; Streptomycin; Model;

机译：30s亚单位;构象转换;引物延伸;Messenger-rna;翻译;链霉素;模型;

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1. MUTATIONS AT POSITIONS 13 AND/OR 914 IN ESCHERICHIA COLI 16S RIBOSOMAL RNA INTERFERE WITH THE INITIATION OF PROTEIN SYNTHESIS [J] . Pinard R., Brakiergingras L., Payant C. Biochemistry . 1995,第29期

机译：大肠埃希氏菌16S核糖体RNA位置13和/或914的突变会干扰蛋白质合成的启动
2. MUTATIONS OF NON-CANONICAL BASE-PAIRS IN THE 3' MAJOR DOMAIN OF ESCHERICHIA COLI 16 S RIBOSOMAL RNA AFFECT THE INITIATION AND ELONGATION OF PROTEIN SYNTHESIS [J] . Dragon F., Pinard R., Carriere J., Journal of Molecular Biology . 1996,第2期

机译：大肠埃希氏菌16 S核糖体RNA的3'主要区域中非经典碱基对的突变影响蛋白质合成的启动和延长
3. RNA-protein cross-linking in Escherichia coli 30S ribosomal subunits; determination of sites on 16S RNA that are cross-linked to proteins S3, S4, S5, S7, S8, S9, S11, S13, S19 and S21 by treatment with methyl p-azidophenyl acetimidate [J] . Barbara Greuer, Georg St?ffler, Hans B?umert, Nucleic acids research . 1987,第8期

机译：大肠杆菌30S核糖体亚基中的RNA-蛋白质交联;通过用对叠氮基苯基乙酰胺酸甲酯处理确定与蛋白质S3，S4，S5，S7，S8，S9，S11，S13，S19和S21交联的16S RNA上的位点
4. A Coordinated Proteomic Approach Suggests A Conserved Loop on Escherichia coli Ribosomal Protein S12 To Be A protein Binding Interface [C] . Michael Brad Strader, Judson Hervey, Nina Costantino, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2011

机译：协调的蛋白质组学方法表明了大肠杆菌核糖体蛋白S12上的保守回路是蛋白质结合界面
5. Nucleotide modifications in helix 31 (970 loop) of 16s ribosomal RNA of Escherichia coli: Biological roles and identification of inhibitory ligands. [D] . Lamichhane, Tek Narayan. 2009

机译：大肠杆菌16s核糖体RNA的螺旋31（970环）中的核苷酸修饰：生物学作用和抑制性配体的鉴定。
6. Mutation at position 791 in Escherichia coli 16S ribosomal RNA affects processes involved in the initiation of protein synthesis. [O] . W E Tapprich, D J Goss, A E Dahlberg 1989

机译：大肠杆菌16S核糖体RNA中791位的突变影响蛋白质合成起始过程。
7. Mutation at position 791 in Escherichia coli 16S ribosomal RNA affects processes involved in the initiation of protein synthesis. [O] . Tapprich, W E, Goss, D J, Dahlberg, A E 1989

机译：大肠杆菌16S核糖体RNA中791位的突变影响蛋白质合成起始过程。
8. Studies on the Control of Ribosomal Protein Synthesis in Escherichia Coli [R] . Ungar, H. G. 1968

机译：大肠杆菌核糖体蛋白合成控制的研究

MUTATIONS AT POSITIONS 13 AND/OR 914 IN ESCHERICHIA COLI 16S RIBOSOMAL RNA INTERFERE WITH THE INITIATION OF PROTEIN SYNTHESIS

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