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首页> 外文期刊>Canadian journal of microbiology >Enhancement of the antifungal activity of Bacillus subtilis F29-3 by the chitinase encoded by Bacillus circulans chiA gene.
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Enhancement of the antifungal activity of Bacillus subtilis F29-3 by the chitinase encoded by Bacillus circulans chiA gene.

机译:圆芽孢杆菌chiA基因编码的几丁质酶增强枯草芽孢杆菌F29-3的抗真菌活性。

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摘要

Bacillus subtilis F29-3 is an antagonistic bacterium against a wide range of fungal species. In order to determine the effect of chitinase on the antifungal activity of B. subtilis F29-3, a 2.4-kb DNA fragment containing the chiA gene of Bacillus circulans WL-12 was ligated into a shuttle vector pHY300PLK and transformed into B. subtilis F29-3. A bioassay conducted on the culture supernatant showed that, in comparison to the B. subtilis control strain, B. subtilis F29-3 expressing the chiA gene exhibited a greater inhibition of spore germination of Botrytis elliptica, indicating that chitinase could enhance the antifungal function conferred by B. subtilis F29-3.
机译:枯草芽孢杆菌F29-3是对多种真菌物种的拮抗细菌。为了确定几丁质酶对枯草芽孢杆菌F29-3的抗真菌活性的影响,将包含圆形芽孢杆菌WL-12的chiA基因的2.4kb DNA片段连接到穿梭载体pHY300PLK中,并转化到枯草芽孢杆菌F29中。 -3。对培养物上清液进行的生物测定表明,与枯草芽孢杆菌对照菌株相比,表达chiA基因的枯草芽孢杆菌F29-3表现出了更大的对灰葡萄孢菌孢子萌发的抑制作用,表明几丁质酶可以增强赋予的抗真菌功能由枯草芽孢杆菌F29-3。

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