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Inhibition of Listeria monocytogenes by Carnobacterium piscicola in Fresh and Pasteurized Crab Meat

机译:食肉杆菌对新鲜和巴氏杀菌蟹肉中李斯特菌的抑制作用

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The ability of Carnobacterium piscicola to inhibit Listeria monocytogenes at different concentration levels.storage times and temperatures was examined. Twenty-five g samples of fresh and previously pasteurized blue crab meat were placed into separatesterile 59 ml souffle cups, inoculated with 0.1 ml C. piscicola at three different concentration levels (2,4 and 6 log CFU/g) and 0.1 ml (log 2.0 CFU) of L monocytogenes (ratios of 1: 1,2:1 and 3:1 of C. piscicola to L monocytogenes). Fresh crab meat and previously pasteurized crab meatwas stored at4°C or 10°C and sampled until aerobic plate count reached > 7 log CFU/g, which was considered spoilage. At each sampling time, crab meat inoculated with both organisms was plated on modified LactobacilliusdeMan Rogosa Sharpe (pH 7) and modified oxford agar (MOX). Uninoculated crab meat was plated on trypticase soy agar and meat inoculated with only L monocytogenes was plated on MOX. In both fresh and pasteurized crab meat, regardless of the inoculation levels, L monocytogenes and C. piscicola followed similar growth trends. C. piscicola reduced growth of L monocytogenes in pasteurized crab meat after 14 day at 4°C and 10 day at 10°C (P < 0.05).
机译:考察了食肉杆菌对不同浓度,贮藏时间和温度的单核细胞增生李斯特菌的抑制能力。将25克新鲜的和事先经过巴氏消毒的蓝蟹肉样品分别放入无菌的59 ml蛋奶酥奶杯中,分别接种0.1 ml三种浓度(2,4和6 log CFU / g)的C. piscicola和0.1 ml(log L单核细胞增生李斯特菌(比C. piscicola 1:1:1,2:1和3:1的比例)。将新鲜的蟹肉和先前经过巴氏消毒的蟹肉分别存储在4°C或10°C并取样,直到好氧板数达到> 7 log CFU / g,这被认为是腐败的。在每个采样时间,将接种两种生物的蟹肉接种在改良的LactobacilliusdeMan Rogosa Sharpe(pH 7)和改良的牛津琼脂(MOX)上。将未接种的蟹肉接种于胰蛋白酶大豆琼脂上,仅接种单核细胞增生李斯特氏菌的肉接种于MOX。在新鲜和巴氏消毒的蟹肉中,无论接种水平如何,单核细胞增生李斯特菌和C. piscicola都遵循相似的生长趋势。在4°C下14天和10°C下10天后,C。piscicola降低了巴氏灭菌蟹肉中L单核细胞增生李斯特氏菌的生长(P <0.05)。

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