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Evaluation of Process Control to Prevent Contamination of Beef with Non-0157 Shiga Toxin-producing Escherichia coli (STEC) in U.S. Export Abattoirs in Honduras and Nicaragua

机译:评估在洪都拉斯和尼加拉瓜的美国出口屠宰场中防止产生非-0157志贺毒素的大肠杆菌污染牛肉的过程控制

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摘要

The objectives of this project were to determine (1) the prevalence of non-0157 STEC on beef hides and carcasses in export abattoirs in Honduras (Plant A) and Nicaragua (Plant B) and (2) whether current practices and interventions controlled final carcass contamination. Samples were collected on the foreshanks from the hides, at pre-evisceration and after application of an antimicrobial treatment. In Plant A, 23.3% (7/30) of hides contained at least one STEC serogroup, whereas in Plant B, 90.0% (45/50) tested positive. Pre-evisceration samples had a prevalence of 6.7% (2/30) for Plant A and of 0% for Plant B. No STEC were detected after antimicrobial intervention. Serogroups 026,045, and 0121 were the most prevalent in plant A, with frequencies of 27/75 (36.0%), 24/75 (32.0%), and 18/75 (24.0%), respectively. In Plant B, 026 and 0121 were predominant, with 47.5% (38/80) and 46.3% (37/80), respectively. STEC were present on the hides, but current hygienic practices and interventions effectively controlled them and reduced final carcass contamination.
机译:该项目的目标是确定(1)洪都拉斯(工厂A)和尼加拉瓜(工厂B)出口屠宰场的牛肉皮和屠体中非0157 STEC的患病率,以及(2)当前的做法和干预措施是否控制了最终屠体污染。在去内脏之前和进行抗菌处理后,从皮革的前腿上收集样品。在工厂A中,23.3%(7/30)的皮革含有至少一个STEC血清群,而在工厂B中,90.0%(45/50)的皮革呈阳性。工厂A的去内脏样品的患病率为6.7%(2/30),工厂B的去内脏样品的患病率是0%。抗菌干预后未检测到STEC。血清群026,045和0121在植物A中最为流行,频率分别为27/75(36.0%),24/75(32.0%)和18/75(24.0%)。在植物B中,以026和0121为主要成分,分别为47.5%(38/80)和46.3%(37/80)。 STEC存在于生皮上,但是当前的卫生习惯和干预措施有效地控制了它们并减少了最终car体污染。

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