...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Food Control >Recent advances in ochratoxin A-producing fungi detection based on PCR methods and ochratoxin A analysis in food matrices.
【24h】

Recent advances in ochratoxin A-producing fungi detection based on PCR methods and ochratoxin A analysis in food matrices.

机译：基于PCR方法和食品基质中曲霉毒素A分析的曲霉毒素A真菌检测技术的最新进展。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Ochratoxin A (OTA), a mycotoxin produced by various Aspergillus and Penicillium strains under diverse environmental conditions, has been found as a common contaminant of wide variety of cereals, dried fruits, spices, coffee and fermented beverages. Due to its widespread on such a large variety of agricultural commodities and the potential health risks, mainly toward humans, prompt detection is important. To prevent OTA contamination in foodstuffs, recently several methods mostly based on PCR-based assays have been developed for identifying and quantifying OTA-producing fungi in food samples. PCR including its different formats remains the technique of choice, thanks to its ability to detect even small amounts of fungal DNA in raw materials and processed foods. In order to meet food safety concerns and official legislated regulations, analytical techniques have been reported for OTA detection. Although most validated methods are chromatographic techniques, alternatives strategies are emerging and novel technologies using antibodies have been proposed, such as immunoassays, immunosensors and lateral-flow devices. Aptamers recently selected against OTA seem a promising tool and have been used these last years in bioanalytical methods for OTA detection, in electrochemical and optical techniques. In this review, we discussed innovative analytical methods that have emerged in recent years for the detection of ochratoxigenic fungi and OTA in food samples. All rights reserved, Elsevier.
机译：ch曲霉毒素A（OTA）是多种曲霉菌和青霉菌菌株在不同环境条件下产生的霉菌毒素，已被发现是多种谷物，干果，香料，咖啡和发酵饮料。由于其广泛存在于如此多种多样的农产品中，并且存在潜在的健康风险，主要是对人类，因此及时发现非常重要。为了防止食品中的OTA污染，最近已经开发了几种主要基于PCR分析的方法来鉴定和定量食品样品中产生OTA的真菌。包括其不同格式的PCR仍然是首选技术，这归功于它能够检测原材料和加工食品中的少量真菌DNA。为了解决食品安全问题和官方立法规定，已报告了用于OTA检测的分析技术。尽管大多数经过验证的方法是色谱技术，但替代策略正在出现，并且提出了使用抗体的新技术，例如免疫测定，免疫传感器和侧流设备。最近针对OTA选择的适体似乎是一种有前途的工具，并且最近几年已在用于OTA检测的生物分析方法，电化学和光学技术中使用。在这篇综述中，我们讨论了近年来出现的创新分析方法，用于检测食物样品中的产毒毒素和OTA。保留所有权利，Elsevier。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号