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Effect of nitrogen and carbon sources on lipase production by Penicillium aurantiogriseum

机译：氮和碳源对金黄色青霉生产脂肪酶的影响

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摘要

A wild fungal strain isolated from soybean oil and identified as Penicillium aurantiogriseum initially presented a volumetric lipase activity of 0.4 U/mL in submerged culture in a medium containing 0.5 % yeast extract and 1 % olive oil. Studies were undertaken to improve lipase production. The effect of nitrogen source was studied by adding casein peptone, meat peptone, yeast extract or ammonium sulfate to a medium containing potassium nitrate and other mineral salts. The best yield, of 13 U/mL after 72 h, was obtained with the medium supplemented with ammonium sulfate. With the ammonium sulfate concentration increased to double the C/N ratio from 2.5 to 5, a lipolytic activity of 18 U/mL was obtained. Olive, corn, soy and sunflower oils were tested as carbon sources in this medium, with olive oil at 1 % giving a lipolytic activity of 25 U/mL after 48 h, the highest yield obtained in this study. Enzyme production was best at 29 degreesC, within a range tested from 26 to 32 degreesC. These results are promising because this strain produces lipase in an inexpensive inorganic medium and,,we succeeded in increasing the lipolytic activity 62-fold over the initial values obtained with the non-optimized medium.
机译：从大豆油中分离出来的一种野生真菌菌株，被鉴定为aurantiogriseum青霉，在含有0.5％酵母提取物和1％橄榄油的培养基中，在浸没培养中的体积脂肪酶活性最初为0.4 U / mL。进行了研究以改善脂肪酶的产生。通过将酪蛋白p，肉蛋白ept，酵母提取物或硫酸铵添加到含有硝酸钾和其他无机盐的培养基中来研究氮源的作用。在添加硫酸铵的培养基中，72小时后的最佳产量为13 U / mL。随着硫酸铵浓度的增加，C / N比从2.5增加到5，两倍，脂解活性达到18 U / mL。在这种培养基中，以橄榄油，玉米，大豆和葵花籽油为碳源进行了测试，其中48％的橄榄油在1％的脂解活性为25 U / mL时，获得了最高的收率。在26到32摄氏度的测试范围内，酶的产生在29摄氏度下最好。这些结果令人鼓舞，因为该菌株在廉价的无机介质中产生脂肪酶，而且我们成功地将脂解活性提高了非优化介质所获得的初始值的62倍。

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