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Purification and characterization of desmutagenic components extracted from sweet potato leaves

机译：甘薯叶中提取的致突变成分的纯化与表征

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摘要

A major desmutagenic factor, purified from water extract of sweet potato (Ipomoea batatas Lam, CN1489-89) leaves by ultrafiltration and gel filtration, and identified using Salmonella typhimurium TA98, was found to have a molecular weight of 269,000and to be made up of protein (42.18 percent) and sugar (57.40 percent). It also exhibited an absorption peak at 280 nm with a small shoulder in the range of 320-330 nm, suggesting that it might be a glycoprotein. The antimutagenicity of the novel substance was studied against 2-amino-3-methylimidazo-[4,5-f]-quinoline (IQ) and its activated metabolites. The desmutagenicity of the novel substance against lQ and its activated metabolites were 58 and 63 percent, respectively and the induced mutation frequency reduced from 5,500 X 10~(-9) to 3,400 X 10~(-9) reflecting a 38 percent bio-antimutagenicity at the same concentration of 0.05 mg. It was found that the suppressive effect of the desmutagenic factor on mutation caused by IQ and its activated metabolites could be ascribed to both desmutagenicity and bio-antimutagenicity, although the former was higher than the latter.
机译：发现主要的诱变因子是通过超滤和凝胶过滤从甘薯（Ipomoea batatas Lam，CN1489-89）的水提物中提纯的，并用鼠伤寒沙门氏菌TA98鉴定出，其分子量为269,000，由蛋白质（42.18％）和糖（57.40％）。它还在280 nm处显示吸收峰，并在320-330 nm范围内有一个小的肩峰，表明它可能是糖蛋白。研究了该新型物质对2-氨基-3-甲基咪唑-[4,5-f]-喹啉（IQ）及其活化代谢产物的抗诱变性。新物质对lQ及其活化代谢产物的去突变性分别为58％和63％，诱导的突变频率从5500 X 10〜（-9）降至3,400 X 10〜（-9），反映了38％的生物抗突变性。在相同的浓度0.05毫克。研究发现，去突变因子对智商及其活化代谢产物引起的突变的抑制作用可归因于去突变性和生物抗突变性，尽管前者高于后者。

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