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首页> 外文期刊>Forest Pathology >Genetic characterization of Botrytis cinerea isolates collected from pine and eucalyptus nurseries in Bio-Bio Region, Chile
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Genetic characterization of Botrytis cinerea isolates collected from pine and eucalyptus nurseries in Bio-Bio Region, Chile

机译:智利Bio-Bio地区从松树和桉树苗圃中收集的灰葡萄孢菌的遗传特征

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摘要

A total of 55 Botrytis cinerea isolates collected from Pinus radiata and Eucalyptus globulus plants cultivated in nurseries located in the Bio-Bio Region, Chile, as well as isolates collected from native plants such as Rubus spp and Aristotelia chilensis located near the nurseries were genetically characterized. All isolates carried the Bc-hch2 allele, thus belonging to genetic Group II, which is now referred to as B.cinerea. Genotyping based on the presence of transposons Boty and Flipper showed differences between isolates related to the plant host. Thus, transposa isolates (containing both transposons) were detected in P.radiata and E.globulus, while vacuma isolates (containing neither transposon) were detected in all plants except E.globulus. Notably, boty isolates (containing just the Boty transposon) were detected at high frequencies in all plant hosts. Analyses to detect mutations involved in resistance to fungicides such as benzimidazoles (BZ), dicarboximides and QoIs also showed differences in the studied isolates. Isolates collected from E.globulus were shown to carry mutations for all tree fungicides, while those collected from P.radiata presented mutations involved in resistance to BZ only. Isolates collected from native plant hosts did not carry any of the mutations analysed.
机译:对从智利Bio-Bio地区的苗圃中种植的辐射松和桉树植物中收集的总共55株灰葡萄孢分离株,以及在苗圃附近的天然植物如悬钩子属和智利马兜铃属的分离株进行了遗传鉴定。所有分离株均携带Bc-hch2等位基因,因此属于基因组II,现在称为B.cinerea。根据转座子Boty和Flipper的存在进行基因分型表明与植物宿主相关的分离株之间存在差异。因此,在辐射假单胞菌和球形大肠杆菌中检测到转座分离物(包含两个转座子),而在除了球形大肠杆菌之外的所有植物中均检测到真空分离株(不包含转座子)。值得注意的是,在所有植物宿主中都以高频率检测到了boty分离株(仅包含Boty转座子)。分析检测与抗真菌剂(例如苯并咪唑(BZ),二甲酰亚胺和QoIs)有关的突变的分析也显示了所研究菌株的差异。从球菌中分离出的分离物被证明带有所有树木杀真菌剂的突变,而从辐射松中分离出的分离物仅表现出对BZ抗性的突变。从天然植物宿主收集的分离株未携带任何经分析的突变。

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