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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Attenuation of oxidative stress, inflammation, and endothelial dysfunction in hypercholesterolemic rabbits by allicin
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Attenuation of oxidative stress, inflammation, and endothelial dysfunction in hypercholesterolemic rabbits by allicin

机译：大蒜素可减轻高胆固醇血症兔的氧化应激，炎症和内皮功能障碍

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摘要

Allicin, the active substance of garlic, exerts a broad spectrum of pharmacological activities and is considered to have potential therapeutic applications. The present study was designed to investigate the possible beneficial effects of allicin against oxidative stress, inflammation, and endothelial dysfunction in hypercholesterolemic rabbits. Male New Zealand white rabbits were used in this study. Rabbits randomly received 1 of the following treatments: normal chow diet for 4 weeks, 1% high cholesterol diet (HCD), HCD plus allicin (10 mg/kg/day), or HCD plus atorvastatin (10 mg/kg/day). Blood samples were collected at the end of experimental diets for measurement of serum total cholesterol (TC), triglycerides (TGs), high-density lipoprotein cholesterol (HDL-C), C-reactive protein (CRP), malondialdehyde (MDA), reduced glutathione (GSH), and superoxide dismutase (SOD). In addition, the aorta was removed for measurement of vascular reactivity, histopathological changes, intima/media (I/M) ratio, and immunohistochemical staining of both tumor necrosis-alpha (TNF-alpha) and nuclear factor (NF)-kappa B. HCD induced significant increases in serum TC, TGs, low-density lipoprotein cholesterol (LDL-C), CRP, and MDA. Moreover, HCD caused significant decrease in serum GSH and SOD. In addition, aortic relaxation response to acetylcholine (ACh) was impaired. Immunohistochemical staining of aortic specimens from HCD-fed rabbits revealed high expression levels of both TNF-alpha and the oxidantinduced transcription factor, NF-kappa B. Allicin supplementation significantly decreased serum MDA and CRP, increased serum HDL-C, GSH, and SOD levels while nonsignificantly affecting HCD-induced elevations in serum TC and LDL-C. Additionally, allicin significantly protected against HCD-induced attenuation of rabbit aortic endothelium-dependent relaxation to ACh and elevation in I/M ratio. This effect was confirmed by histopathological examination of the aorta. Moreover, allicin has substantially beneficial effects on aortic expression of TNF-alpha and NF-kappa B compared with HCD-fed rabbits. In conclusion, these findings demonstrate that allicin may be useful in reducing oxidative stress, inflammation, vascular dysfunction, and the aortic pathology in hypercholesterolemic rabbits.
机译：大蒜素的活性物质大蒜素具有广泛的药理活性，被认为具有潜在的治疗应用。本研究旨在调查大蒜素对高胆固醇血症兔的氧化应激，炎症和内皮功能障碍的可能有益作用。这项研究使用了雄性新西兰白兔。兔随机接受以下治疗方法之一：正常饮食4周，1％高胆固醇饮食（HCD），HCD加大蒜素（10 mg / kg /天）或HCD加阿托伐他汀（10 mg / kg /天）。在实验饮食结束时收集血样，以测量血清总胆固醇（TC），甘油三酸酯（TGs），高密度脂蛋白胆固醇（HDL-C），C反应蛋白（CRP），丙二醛（MDA）谷胱甘肽（GSH）和超氧化物歧化酶（SOD）。此外，移除主动脉以测量肿瘤坏死-α（TNF-alpha）和核因子（NF）-κB的血管反应性，组织病理学变化，内膜/中膜（I / M）比率以及免疫组织化学染色。 HCD导致血清TC，TG，低密度脂蛋白胆固醇（LDL-C），CRP和MDA显着增加。而且，HCD导致血清GSH和SOD明显降低。此外，对乙酰胆碱（ACh）的主动脉舒张反应受损。喂食HCD的兔子的主动脉标本的免疫组织化学染色显示TNF-α和氧化剂诱导的转录因子NF-κB均高表达。补充大蒜素可显着降低血清MDA和CRP，增加血清HDL-C，GSH和SOD水平而对HCD引起的血清TC和LDL-C升高没有显着影响。此外，大蒜素显着保护了HCD诱导的兔主动脉内皮依赖性ACh松弛至ACh的衰减和I / M比的升高。通过主动脉的组织病理学检查证实了该作用。此外，与喂食HCD的兔子相比，大蒜素对TNF-α和NF-κB的主动脉表达具有实质性的有益作用。总之，这些发现表明，大蒜素可能有助于减少高胆固醇血症兔的氧化应激，炎症，血管功能障碍和主动脉病变。

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