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首页> 外文期刊>Gene expression >Expression of septin 3 isoforms in human brain.
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Expression of septin 3 isoforms in human brain.

机译：septin 3亚型在人脑中的表达。

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摘要

Septin 3 is a novel member of the septin subfamily of GTPase domain proteins. Human septin 3 was originally cloned during a screening of genes expressed in human teratocarcinoma cells induced to differentiate with retinoic acid. Alternative splicing of the septin 3 gene transcript produces two isoforms, A and B, in the human brain, though their regional expression and physiological function remain to be determined. The purpose of the present study was to identify the expression patterns of human septin 3 isoforms in normal human brain and a human neuroblastoma cell line, SH-SY5Y, after retinoic acid-induced differentiation. The expression and distribution patterns of septin 3 isoforms A and B were similar and resembled that of another septin, CDCrel-1. Septin 3A and 3B were expressed in normal human brain in a region-specific manner, with the highest level in the temporal cortex and hippocampus and the lowest level in the brainstem regions. Prominent immunoreactivity was observed diffusely in the neocortices in association with neuropils and punctate structures suggestive of synaptic junctions. Immunoprecipitation studies revealed that septin 3A, 3B, and CDCrel-1 form a complex in the frontal cortex of human brain. These findings, taken together, suggest that septin 3A and 3B, along with CDCrel-1, play some fundamental role(s) in synaptogenesis and neuronal development.
机译：Septin 3是GTPase域蛋白septin亚家族的新成员。人类Septin 3最初是在筛选人视网膜癌细胞中表达的基因时克隆的，该细胞被视黄酸诱导分化。 septin 3基因转录物的可变剪接在人脑中产生两种同工型A和B，尽管它们的区域表达和生理功能仍有待确定。本研究的目的是确定视黄酸诱导的分化后正常人脑和人成神经细胞瘤细胞系SH-SY5Y中人septin 3同工型的表达模式。 septin 3同工型A和B的表达和分布模式相似，与另一个septin CDCrel-1相似。 Septin 3A和3B在正常人脑中以区域特定的方式表达，在颞叶皮质和海马中最高，而在脑干区域中最低。在新皮层中广泛观察到显着的免疫反应性，提示神经突触和点状结构与突触连接有关。免疫沉淀研究显示septin 3A，3B和CDCrel-1在人脑额叶皮层中形成复合物。这些发现加在一起表明Septin 3A和3B以及CDCrel-1在突触形成和神经元发育中起着基本作用。

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