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首页> 外文期刊>Fish & Shellfish Immunology >The role of oncoprotein NM23 gene from Exopalaemon carinicauda is response to pathogens challenge and ammonia-N stress
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The role of oncoprotein NM23 gene from Exopalaemon carinicauda is response to pathogens challenge and ammonia-N stress

机译：鬼虾Exopalaemon carinicauda的癌蛋白NM23基因的作用是响应病原体攻击和氨氮胁迫

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摘要

Oncoprotein NM23, as a family of genes encoding the nucleoside diphosphate (NDP) kinase, plays important roles in bioenergetics, DNA replication, differentiation and tumor metastasis. In this study, a full-length cDNA of NM23 (designated EcNM23) was cloned from Exopalaemon carinicauda by using rapid amplification of cDNA ends (RACE) approaches. The full-length cDNA of EcNM23 was 755 bp, which contains an open reading frame (ORF) of 518 bp, encoding a 175 amino-acid polypeptide with the predicted molecular weight of 19.60 kDa and estimated isoelectric point of 7.67. The deduced amino acid sequence of EcNM23 shared high identity (86%-93%) with that of other crustaceans. a NDP kinase super family signature was identified in E. carinicauda EcNM23. Quantitative real-time RT-qPCR analysis indicated that EcNM23 was expressed in all the examined tissues with the high expression level in hemocytes and ovary. The EcNM23 expression in immune-related tissues changed rapidly and reached peak at different time after pathogens (Vibrio parahaemolyticus and WSSV) challenge and ammonia-N stress treatment. The results suggested that EcNM23 might be associated with the immune defenses to pathogens infection and ammonia-N stress in E. carinicauda. (C) 2015 Elsevier Ltd. All rights reserved.
机译：癌蛋白NM23是编码二磷酸核苷（NDP）激酶的基因家族，在生物能，DNA复制，分化和肿瘤转移中起着重要作用。在这项研究中，通过快速扩增cDNA末端（RACE）方法，从Exopalaemon carinicauda克隆了NM23的全长cDNA（命名为EcNM23）。 EcNM23的全长cDNA为755 bp，包含一个518 bp的开放阅读框（ORF），编码一个175个氨基酸的多肽，预测分子量为19.60 kDa，估计等电点为7.67。推导的EcNM23氨基酸序列与其他甲壳类动物具有较高的同一性（86％-93％）。在Cari​​nicauda EcNM23中鉴定了NDP激酶超家族标志。实时定量RT-qPCR分析表明，EcNM23在所有检查的组织中均有表达，在血细胞和卵巢中的表达水平很高。病原体（副溶血性弧菌和WSSV）攻击和氨氮胁迫后，免疫相关组织中的EcNM23表达迅速变化并在不同时间达到峰值。结果表明，EcNM23可能与大肠杆菌中病原体感染和氨氮胁迫的免疫防御有关。 （C）2015 Elsevier Ltd.保留所有权利。

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