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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Thermodynamic stoichiometry of Na+-coupled glutathione transport.
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Thermodynamic stoichiometry of Na+-coupled glutathione transport.

机译：Na +偶联谷胱甘肽转运的热力学化学计量。

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摘要

Ambiguity exists with respect to mechanisms of glutathione (GSH) transport and the molecular identity of GSH transporters. Empirical and theoretical limitations have hindered functional and molecular characterizations. Published literature referring to the isolation and molecular identification of Na+-coupled GSH transporters that mediate the cellular uptake of GSH is highly debated. Whereas a number of functional and kinetic reports of this putative symport mechanism exist, the hypothetical transmembrane Na+-coupled GSH transporter protein or the genetic message encoding it has not been isolated. Theoretical thermodynamic calculations to support the concept of secondary active GSH transport and to rationalize accounts of physical-kinetic measurements describing Na+-coupled cellular GSH uptake were performed. The adequacy of requisite energy and stoichiometric conservation of the separate electrical and chemical components of a Na+ gradient in maintaining a high cellular accumulation gradient for GSH was examined through a purely phenomenological perspective. Dependent on the biological context, the energetic coupling between Na+ and GSH cotransport may occur at ratios from 1:1 to 3:1. Molecular identification of specific transporters responsible for cellular Na+-coupled GSH uptake will facilitate determination of their relative contribution to the overall plasma membrane resting potential. In tissues with a high GSH concentration relative to their extracellular millieu, particularly in pathologies of cystic fibrosis and dry eye syndromes, large energy coupling ratios in cotransport of Na+ and GSH may be expected. Na+-coupled GSH transport may play an important role in disease onset and (or) progression, or treatment modalities thereof.
机译：谷胱甘肽（GSH）转运的机制和GSH转运蛋白的分子身份存在歧义。经验和理论上的限制阻碍了功能和分子表征。涉及介导GSH细胞摄取的Na +偶联GSH转运蛋白的分离和分子鉴定的公开文献引起了广泛的争论。尽管有许多关于这种假定的共生机制的功能和动力学报告，但尚未分离出假设的跨膜Na +偶联的GSH转运蛋白或编码它的遗传信息。进行了理论热力学计算，以支持次级主动GSH转运的概念，并使描述Na +偶联的细胞GSH吸收的物理动力学测量值合理化。通过纯粹的现象学角度检查了维持GSH的高细胞积累梯度时，Na +梯度的单独电气和化学成分的必要能量是否充足以及化学计量守恒。取决于生物学背景，Na +和GSH共转运之间的能量耦合可能以1：1至3：1的比例发生。分子识别负责细胞Na +偶联的GSH摄取的特定转运蛋白将有助于确定它们对总体质膜静息潜力的相对贡献。在相对于其细胞外毫微球具有高GSH浓度的组织中，尤其是在囊性纤维化和干眼症候群的病理学中，可以预期在Na +和GSH的共转运中能量耦合比很大。 Na +偶联的GSH转运可能在疾病发作和（或）进展或其治疗方式中起重要作用。

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