...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Fish Physiology and Biochemistry >Lack of growth hormone-releasing peptide-6 action on in vivo and in vitro growth hormone secretion in sexually immature and grass carp (Ctenopharyngodon idellus)
【24h】

Lack of growth hormone-releasing peptide-6 action on in vivo and in vitro growth hormone secretion in sexually immature and grass carp (Ctenopharyngodon idellus)

机译：缺乏生长激素释放肽-6对有性未成熟和草鱼（Ctenopharyngodon idellus）体内和体外生长激素分泌的作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Studies in mammals have shown that synthetic Met-enkephalin derivatives, called growth hormone-releasing peptides (GHRPs), stimulate growth hormone (GH) release. In the present study, GHRP-6 action on GH secretion was examined in vivo and in vitro in sexually immature grass carp. GHRP-6 injected intraperitoneally had no influences on serum GH levels in juvenile grass carp. Following intraperitonal injection of GHRP-6 and dopamine (DA) or cysteamine hydrochloride (CSH), alone and in combination into juvenile grass carp, DA and CSH were effective in elevating serum GH levels, but GHRP-6 was not effective in this respect; in addition, the synergistic action of GHRP-6 and DA or CSH on GH secretion was not seen. In this work, we had adapted and validated a perifusion system and a culture system for GH regulation studies. In a perifusion system, GHRP-6 ( 1000 to 0.1 nM), GHRP-6 (0.1 to 1000 nM), GHRP-6 (1 muM), and Hexarelin (an analog of GHRP, 1 muM) had no action on GH release from juvenile grass carp pituitary fragments or cells. Under static incubation conditions, GHRP-6 was inactive on GH release from juvenile grass carp pituitary fragments after 1 h and 6 h incubation, but human growth hormone-releasing hormone ( hGHRH; 1 to 100 nM) as positive control could stimulate GH release in a dose-dependent manner. Furthermore, when GHRP-6 (100 nM) in combination static incubation with neuropeptides [e.g., hGHRH (100 nM), salmon gonadotropin-releasing hormone analogue (sGnRH-A) (100 nM), or D-Ala(6), Pro(9)-NEt- luteinizing hormone-releasing hormone (D-Ala(6), Pro(9)-NEt-LHRH, LHRH-A) (100nM)], GHRP-6 did not strengthen GH secretion actions of neuropeptides, and at the same time neuropeptides also did not modify the effects of GHRP-6 on GH secretion. The present results obtained using in vivo and in vitro techniques adapted for GH regulation studies show that GHRP-6 does not function as a GH-releasing factor in juvenile grass carp as it does in tilapia, amphibians, chickens, and mammals.
机译：哺乳动物的研究表明，合成的Met-脑啡肽衍生物称为生长激素释放肽（GHRP），可刺激生长激素（GH）释放。在本研究中，在性成熟的草鱼体内和体外检查了GHRP-6对GH分泌的作用。腹膜内注射GHRP-6对未成年草鱼的血清GH水平没有影响。腹膜内注射GHRP-6和多巴胺（DA）或半胱胺盐酸盐（CSH）后，DA和CSH单独或与幼鱼联合使用可有效提高血清GH水平，但GHRP-6在这方面无效。此外，未观察到GHRP-6和DA或CSH对GH分泌的协同作用。在这项工作中，我们已经调整并验证了用于GH调节研究的灌注系统和培养系统。在灌注系统中，GHRP-6（1000至0.1 nM），GHRP-6（0.1至1000 nM），GHRP-6（1μM）和Hexarelin（GHRP的类似物1μM）对GH释放没有作用来自幼草鱼的垂体碎片或细胞。在静态温育条件下，GHRP-6在温育1 h和6 h后对少年草鱼垂体碎片中GH的释放没有活性，但作为人类生长激素释放激素（hGHRH; 1至100 nM）的阳性对照可以刺激GHRP-6的释放。剂量依赖性的方式。此外，当GHRP-6（100 nM）与神经肽[例如hGHRH（100 nM），鲑鱼促性腺激素释放激素类似物（sGnRH-A）（100 nM）或D-Ala（6）结合静态孵育时，Pro （9）-促黄体生成素释放激素（D-Ala（6），Pro（9）-NEt-LHRH，LHRH-A）（100nM）]，GHRP-6不能增强神经肽的GH分泌作用，并且同时神经肽也没有改变GHRP-6对GH分泌的影响。使用适用于GH调节研究的体内和体外技术获得的当前结果表明，GHRP-6在少年草鱼中不像在罗非鱼，两栖动物，鸡和哺乳动物中起GH释放因子的作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号