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首页> 外文期刊>Frontiers in bioscience: a journal and virtual library >Initial signaling response to acute exercise bout is similar in hearts of rats bred for divergent exercise capacities.
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Initial signaling response to acute exercise bout is similar in hearts of rats bred for divergent exercise capacities.

机译：在因运动能力差异而饲养的大鼠心脏中，对急性运动发作的初始信号反应相似。

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摘要

Rats artificially selected as low capacity runners (LCR) exhibit features of the metabolic syndrome, and blunted exercise training-induced cardiac hypertrophy compared with high capacity runners (HCR). We tested the hypothesis that the divergent cardiac phenotypes may be due to diminished activation of signaling proteins in LCR vs HCR rats. LCR (n=18) and HCR (n=18) rats were randomly assigned to acute exercise or control groups. Ten minutes after a 10-min bout of high intensity treadmill exercise, rats were euthanized, and left ventricles (LV) were harvested. LV homogenates were immunoblotted for phosphorylated and total levels of extracellular regulated kinase (ERK1/2), c-Jun N-terminal kinase (JNK), p38, Akt, S6, and the ribosomal S6 protein kinases S6K and p90RSK. Alterations in protein ubiquitination were examined as an index of protein turnover. In LCR and HCR rats, S6 was activated to a similar extent after exercise (5-fold vs control), as were JNK1/2, p38, and ERK1/2 (each 1.5-fold). Exercise significantly reduced ubiquitination of some proteins, suggesting diminished post-exercise protein degradation. That no significant LCR/HCR differences were observed 10-min post-exercise in the signaling pathways studied herein suggests that the source of the differing cardiac phenotypes in LCR/HCR rats may involve differing activation times and/or other signaling pathways.
机译：人工选择为低容量跑步者（LCR）的大鼠表现出代谢综合征的特征，与高容量跑步者（HCR）相比，运动训练导致的心脏肥大变钝。我们测试了这样的假说，即不同的心脏表型可能是由于LCR和HCR大鼠中信号蛋白的激活减少所致。将LCR（n = 18）和HCR（n = 18）大鼠随机分为急性运动组或对照组。高强度跑步机运动10分钟后十分钟，对大鼠实施安乐死，并收获左心室（LV）。 LV匀浆免疫印迹的磷酸化和总水平的细胞外调节激酶（ERK1 / 2），c-Jun N端激酶（JNK），p38，Akt，S6和核糖体S6蛋白激酶S6K和p90RSK。蛋白质泛素化的变化作为蛋白质更新的指标进行了检查。在LCR和HCR大鼠中，运动后S6的激活程度相似（相对于对照为5倍），JNK1 / 2，p38和ERK1 / 2也是如此（均为1.5倍）。锻炼可显着减少某些蛋白质的泛素化，表明运动后蛋白质降解减少。在本文研究的信号通路中，运动后10分钟未观察到明显的LCR / HCR差异，这表明LCR / HCR大鼠中不同心脏表型的来源可能涉及不同的激活时间和/或其他信号通路。

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