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首页> 外文期刊>Marine biotechnology >Over-expression of the peroxisomal ascorbate peroxidase (SbpAPX) gene cloned from halophyte Salicornia brachiata confers salt and drought stress tolerance in transgenic tobacco.
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Over-expression of the peroxisomal ascorbate peroxidase (SbpAPX) gene cloned from halophyte Salicornia brachiata confers salt and drought stress tolerance in transgenic tobacco.

机译：从盐生植物Salicornia brachiata克隆的过氧化物酶体抗坏血酸过氧化物酶（SbpAPX）基因的过表达赋予转基因烟草耐盐和干旱的耐受性。

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摘要

Salicornia brachiata Roxb., an extreme halophyte, is a naturally adapted higher plant model for additional gene resources to engineer salt tolerance in plants. Ascorbate peroxidase (APX) plays a key role in protecting plants against oxidative stress and thus confers abiotic stress tolerance. A full-length SbpAPX cDNA, encoding peroxisomal ascorbate peroxidase, was cloned from S. brachiata. The open reading frame encodes for a polypeptide of 287 amino acid residues (31.3-kDa protein). The deduced amino acid sequence of the SbpAPX gene showed characteristic peroxisomal targeting sequences (RKRAI) and a C-terminal hydrophobic region of 39 amino acid residues containing a transmembrane domain (TMD) of 23 amino acid residues. Northern blot analysis showed elevated SbpAPX transcript in response to salt, cold, abscisic acid and salicylic acid stress treatments. The SbpAPX gene was transformed to tobacco for their functional validation under stresses. Transgenic plants over-expressing SbpAPX gene showed enhanced salt and drought stress tolerance compared to wild-type plants. Transgenic plants showed enhanced vegetative growth and germination rate both under normal and stressed conditions. Present study revealed that the SbpAPX gene is a potential candidate, which not only confers abiotic stress tolerance to plants but also seems to be involved in plant growth.
机译：Salicornia brachiata Roxb。是一种极端的盐生植物，是一种自然适应的高等植物模型，具有更多的基因资源来工程化植物的耐盐性。抗坏血酸过氧化物酶（APX）在保护植物免受氧化胁迫中起关键作用，因此赋予非生物胁迫耐受性。从臂状链球菌中克隆了编码过氧化物酶体抗坏血酸过氧化物酶的全长SbpAPX cDNA。开放阅读框编码287个氨基酸残基的多肽（31.3-kDa蛋白）。推导的SbpAPX基因的氨基酸序列显示了特征性的过氧化物酶体靶向序列（RKRAI）和39个氨基酸残基的C端疏水区，其中包含23个氨基酸残基的跨膜结构域（TMD）。 Northern印迹分析显示，对盐，冷，脱落酸和水杨酸胁迫处理响应较高的SbpAPX转录物。为了在压力下进行功能验证，将SbpAPX基因转化为烟草。与野生型植物相比，过表达SbpAPX基因的转基因植物表现出增强的盐和干旱胁迫耐受性。转基因植物在正常和胁迫条件下均显示出增强的营养生长和发芽率。目前的研究表明，SbpAPX基因是潜在的候选基因，不仅赋予植物非生物胁迫耐受性，而且似乎与植物生长有关。

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