Identification of a Tcl -like transposon integration site in the genome of the flounder (Platichthys flesus): A novel use of an inverse PCR method

Anita Pocwierz-Kotus; Artur Burzynski; Roman Wenne

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Identification of a Tcl -like transposon integration site in the genome of the flounder (Platichthys flesus): A novel use of an inverse PCR method

机译：比目鱼（Platichthys flesus）基因组中Tcl样转座子整合位点的鉴定：反向PCR方法的新用途

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The inverse PCR method has been developed and applied employed for the identification of the integration sites of the Tcl-like transposons in the genome of the flounder, Platichthys flesus. One Tcl-like insertion instance was recognized and characterized, demonstrating an efficiency of the method for determining of transposon integration sites. The similarity of the sequence flanking transposon (SFT) to reverse transcriptase sequences (RVT) was demonstrated. It is likely that the insertion took place within currently degenerated LINE (long interspersed nuclear elements) retrotransposon.

机译：已经开发了反向PCR方法，并将其用于比目鱼（Platichthys flesus）基因组中Tcl样转座子的整合位点的鉴定。一个Tcl样插入实例被识别和表征，证明了确定转座子整合位点的方法的效率。证明了侧翼转座子（SFT）与逆转录酶序列（RVT）的相似性。该插入很可能发生在当前退化的LINE（长散布的核元素）反转录转座子中。

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《Marine genomics》 |2010年第1期|共6页
作者
Anita Pocwierz-Kotus; Artur Burzynski; Roman Wenne;
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原文格式 PDF
正文语种 eng
中图分类遗传学;
关键词
Tr 1-like transposons; HMM; LINES; Non-LTR retrotransposons;

机译：Tr 1样转座子;HMM;LINES;非LTR逆转座子;

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机译：比目鱼（Platichthys flesus）基因组中Tcl样转座子整合位点的鉴定：反向PCR方法的新用途
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Identification of a Tcl -like transposon integration site in the genome of the flounder (Platichthys flesus): A novel use of an inverse PCR method

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