A REITERATIVE MODE OF DNA SYNTHESIS ADOPTED BY HIV-1 REVERSE TRANSCRIPTASE AFTER A MISINCORPORATION

Ricchetti M; Buc H

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A REITERATIVE MODE OF DNA SYNTHESIS ADOPTED BY HIV-1 REVERSE TRANSCRIPTASE AFTER A MISINCORPORATION

机译：错误导入后，HIV-1逆转录酶采用的DNA合成重复模式。

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Amplification of oligonucleotide repeats is a major cause of variability and instability of genomes, This phenomenon is probably due to an aberration in the copying process of polymerases. We show here that in the presence of MnCl2, mismatch formation commits HIV-1 reverse transcriptase to a new mode of DNA synthesis which generates repetitive products. This activity is distinct from terminal transferase since it requires specific DNA motifs in the template. This mechanisn, which is processive, also works on homologous RNA templates where it generates reiterative products more than 150 nucleotides long. The corresponding mechanism, which involves extensive primer misalignment, is strikingly similar to that postulated for telomerases.

机译：寡核苷酸重复序列的扩增是基因组变异和不稳定性的主要原因。这种现象可能是由于聚合酶复制过程中的异常所致。我们在这里显示了在存在MnCl2的情况下，错配形成使HIV-1逆转录酶进入了一种新的DNA合成模式，该模式会产生重复产物。该活性与末端转移酶不同，因为它需要模板中具有特定的DNA基序。该机制是过程性的，也可用于同源RNA模板上，在该模板上它产生长度超过150个核苷酸的重复产物。涉及广泛的引物错配的相应机制与假定的端粒酶惊人地相似。

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《Biochemistry》 |1996年第47期|共14页
作者
Ricchetti M; Buc H;
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正文语种 eng
中图分类生物化学;
关键词
Simple repetitive dna; Rna-polymerase; Template; Mechanism; Fidelity; Promoter; Replication; Termination; Telomerase; Infidelity;

机译：简单重复DNA;Rna聚合酶;模板;机制;保真度;启动子;复制;终止;端粒酶;不忠;

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专利

1. A REITERATIVE MODE OF DNA SYNTHESIS ADOPTED BY HIV-1 REVERSE TRANSCRIPTASE AFTER A MISINCORPORATION [J] . Ricchetti M, Buc H Biochemistry . 1996,第47期

机译：错误导入后，HIV-1逆转录酶采用的DNA合成重复模式。
2. The HIV-1 reverse transcriptase mutants G190S and G190A, which confer resistance to non-nucleoside reverse transcriptase inhibitors, demonstrate reductions in RNase H activity and DNA synthesis from tRNA(Lys,3) that correlate with reductions in replication efficiency [J] . Wang J., Dykes C., Domaoal R. A., Virology . 2006,第2期

机译：HIV-1逆转录酶突变体G190S和G190A赋予了非核苷逆转录酶抑制剂的抗性，证明RNase H活性的降低和tRNA（Lys，3）的DNA合成与复制效率的降低相关
3. HIV-1 reverse transcriptase mutants resistant to nonnucleoside reverse transcriptase inhibitors do not adversely affect DNA synthesis: pre-steady-state and steady-state kinetic studies. [J] . Domaoal RA, Bambara RA, Demeter LM JAIDS: Journal of acquired immune deficiency syndromes . 2006,第4期

机译：对非核苷类逆转录酶抑制剂具有抗性的HIV-1逆转录酶突变体不会对DNA合成产生不利影响：稳态前和稳态动力学研究。
4. 3D molecular similarity methods: Application to Modeling HIV-1 Reverse Transcriptase Inhibitor Binding [C] . Douglas C. Rohrer, Jordi Mestres, NATO NATO advanced study institute on experimental and computational approaches to structure-based drug design . 1998

机译：3D分子相似性方法：应用于拟合HIV-1逆转录酶抑制剂结合的应用
5. Part I: Synthesis of Cyclobutyl Nucleoside Analogs That Mimic AZT for Inhibition of the K65R HIV-1 Reverse Transcriptase Mutant. Part II: Synthesis and Evaluation of Truncated Triptolide Analogs to Suppress Chronic Inflammation [D] . Bluemling, Gregory Richard 2011

机译：第一部分：模拟AZT抑制K65R HIV-1逆转录酶突变体的环丁基核苷类似物的合成。第二部分：截短雷公藤甲素类似物的合成和评估，以抑制慢性炎症
6. The HIV-1 reverse transcriptase mutants G190S and G190A which confer resistance to non-nucleoside reverse transcriptase inhibitors demonstrate reductions in RNase H activity and DNA synthesis from tRNALys3 that correlate with reductions in replication efficiency [O] . J. Wang, C. Dykes, R.A. Domaoal, -1

机译：HIV-1逆转录酶突变体G190S和G190A赋予了非核苷逆转录酶抑制剂的抗性证明RNase H活性降低和tRNALys合成DNA的减少3与复制效率的降低相关
7. The HIV-1 reverse transcriptase mutants G190S and G190A, which confer resistance to non-nucleoside reverse transcriptase inhibitors, demonstrate reductions in RNase H activity and DNA synthesis from tRNALys, 3 that correlate with reductions in replication efficiency [O] . J. Wang, C. Dykes, R.A. Domaoal, 2006

机译：HIV-1逆转录酶突变体G190S和G190A，其赋予非核苷逆转录酶抑制剂的抗性，证明了RNA酶H活性和从TRNARY的DNA合成的降低，3与复制效率的降低相关

A REITERATIVE MODE OF DNA SYNTHESIS ADOPTED BY HIV-1 REVERSE TRANSCRIPTASE AFTER A MISINCORPORATION

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