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首页> 外文期刊>Matrix biology: Journal of the International Society for Matrix Biology >A novel basal lamina matrix of the stratified epithelium of the ovarian follicle.
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A novel basal lamina matrix of the stratified epithelium of the ovarian follicle.

机译：卵巢卵泡分层上皮的新型基底层基质。

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摘要

Basal laminas are important sheets of specialized extracellular matrix that underlie and surround groups of cells, such as epithelia or endothelia, enabling the cells to orientate their basal/apical polarity and creating a microenvironment for them. Basal laminas can also individually encapsulate whole cells, such as muscle cells, thereby forming a microenvironment but not polarizing the enclosed cells. Other mesenchymal or stromal cells exist with no basal lamina. In the course of studying the bovine follicular basal lamina which underlies the multilayered epithelium of the ovarian follicle, we identified a developmentally regulated novel extracellular matrix (which we call focimatrix for focal intra-epithelial matrix). Focimatrix is composed of basal lamina-like material deposited as plaques or aggregates between the multilayers of the epithelial granulosa cells. The focimatrix does not encapsulate individual or groups of cells and therefore does not form a microenvironment for them. Focimatrix contains collagen type IV subunits alpha1 and alpha2 (but not alpha3-alpha6), and laminin chains alpha1, beta2 and gamma1 (but not alpha2 or beta1), and nidogen-1 and perlecan (but not versican). The amount of focimatrix increases with increasing follicular size, and its appearance precedes the expression by granulosa cells of the enzymes for steroid hormone synthesis, cholesterol side-chain cleavage cytochrome P450 (SCC) and 3beta-hydroxysteroid dehydrogenase (3beta-HSD), in the days preceding ovulation. The expression in granulosa cells of two components examined, nidogen-1 and perlecan, also increases substantially when follicles enlarge to a sufficient size capable of ovulating. Following ovulation the follicular basal lamina is degraded, and presumably focimatrix is too since it is not detected in corpora lutea that develop from the ovulating follicles. During this development the granulosa cells undergo an epithelial-mesenchymal transition (EMT) into luteal cells following ovulation, and substantially increase their expression of steroidogenic enzymes in the process. During EMT epithelial cells lose polarity. Since focimatrix exists on more than one side of the granulosa cells, we propose that it disrupts the polarity induced by the follicular basal lamina in the lead up to ovulation. Hence focimatrix maybe a key part of the follicular/luteal EMT.
机译：基底层是专门的细胞外基质的重要薄片，位于细胞群的上层或周围，例如上皮细胞或内皮细胞，使细胞能够定向其基础/顶极极性并为其创造微环境。基底层也可以单独包裹整个细胞，例如肌肉细胞，从而形成微环境，但不会极化封闭的细胞。存在其他间充质或基质细胞，无基底层。在研究卵巢卵泡多层上皮基础的牛卵泡基底层的过程中，我们鉴定了一种发育受调节的新型细胞外基质（我们称呼为母细胞基质是上皮内局灶性基质）。 Focimatrix由沉积为上皮颗粒细胞多层之间的斑块或聚集体的基底层状材料组成。基质基质不包封单个或一组细胞，因此不为它们形成微环境。 Focimatrix包含IV型胶原蛋白亚基alpha1和alpha2（但不包含alpha3-alpha6），层粘连蛋白链alpha1，beta2和gamma1（但不包含alpha2或beta1），以及nidogen-1和perlecan（但不包含versican）。卵母细胞基质的量随卵泡大小的增加而增加，并且其出现先于颗粒细胞表达类固醇激素合成酶，胆固醇侧链裂解细胞色素P450（SCC）和3β-羟基类固醇脱氢酶（3beta-HSD）的酶。排卵前几天。当卵泡扩大到足以排卵的足够大小时，所检查的两种成分，即nidogen-1和perlecan，在颗粒细胞中的表达也显着增加。排卵后，卵泡基底层被降解，并且据推测也是卵母细胞基质，因为在从卵泡形成的黄体中未检测到它。在此发育过程中，颗粒细胞在排卵后经历上皮-间质转化（EMT）进入黄体细胞，并在此过程中显着增加其类固醇生成酶的表达。在EMT期间，上皮细胞失去极性。由于fociamatrix存在于颗粒细胞的一侧以上，因此我们建议其破坏卵泡基底层诱导的极性，直至排卵。因此，母细胞基质可能是卵泡/黄体EMT的关键部分。

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