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首页> 外文期刊>Matrix biology: Journal of the International Society for Matrix Biology >Expression of serum amyloid A in chondrocytes and myoblasts differentiation and inflammation: possible role in cholesterol homeostasis.
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Expression of serum amyloid A in chondrocytes and myoblasts differentiation and inflammation: possible role in cholesterol homeostasis.

机译：软骨细胞中血清淀粉样蛋白A的表达以及成肌细胞的分化和炎症：可能在胆固醇稳态中发挥作用。

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摘要

Serum amyloid A (SAA) is synthesized by the liver during the acute phase. Local expression of SAA mRNA has been reported also in non-liver cells, a potential local source of SAA protein not related to the systemic acute phase response. SAA function has not been established yet. In the present study, we identified SAA as a protein expressed by chondrocytes and myoblasts in response to inflammatory stimula. In both cell systems, SAA mRNA and protein expression is strongly stimulated by bacterial lipopolysaccharide treatment. SAA mRNA expression is also enhanced during terminal differentiation of cells of the chondrogenic and myogenic lineage; mRNA is barely detectable in prechondrogenic cells and is highly expressed in differentiated hyperthrophic chondrocytes. An increased level of SAA mRNA was also observed in vivo when we compared mRNA extracted from tibiae of 10 day embryos, still fully cartilaginous, with tibiae from 18 day embryos, a stage when the endochondral ossification process has already started. p38 activation, a well-known event of the chondrogenesis signaling cascade, controls expression of SAA in cartilage following inflammatory stimuli. SAA secreted by stimulated chondrocytes is associated with cholesterol. Cholesterol is synthesized by the same chondrocytes and is also increased in inflammatory conditions. A role of SAA in cholesterol homeostasis in chondrocytes is proposed.
机译：血清淀粉样蛋白A（SAA）由肝脏在急性期合成。还已经报道了非肝脏细胞中SAA mRNA的局部表达，这是与全身急性期反应无关的SAA蛋白的潜在局部来源。 SAA功能尚未建立。在本研究中，我们确定了SAA是由软骨细胞和成肌细胞表达的一种蛋白质，可响应炎症刺激。在两种细胞系统中，细菌脂多糖处理均强烈刺激SAA mRNA和蛋白质表达。 SAA mRNA表达在软骨和成肌谱系细胞的终末分化过程中也得到增强。 mRNA在软骨形成前细胞中几乎检测不到，并在分化的高嗜性软骨细胞中高表达。当我们比较仍完全软骨的10天胚胎胫骨提取的mRNA与18天胚胎胫骨提取的mRNA时，体内SAA mRNA的水平也增加了，这是软骨内骨化过程已经开始的阶段。 p38激活是软骨生成信号级联反应的一个众所周知的事件，它控制炎症刺激后软骨中SAA的表达。受刺激的软骨细胞分泌的SAA与胆固醇有关。胆固醇是由相同的软骨细胞合成的，并且在炎症条件下也会增加。提出了SAA在软骨细胞中胆固醇稳态中的作用。

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