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首页> 外文期刊>Methods in cell science: an official journal of the Society for In Vitro Biology >Telomere length measurement by Q-FISH.
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Telomere length measurement by Q-FISH.

机译：通过Q-FISH测量端粒长度。

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摘要

Telomeres are essential functional elements of eukaryotic chromosomes involved in genome stability maintenance. The most important indicator of correct telomere function is telomere length maintenance within the range typical for each species. Telomere length can be estimated by the classical methodology based on Southern blot. However, this methodology is relatively crude and can provide estimate of average telomere length only. To overcome disadvantages of classical telomere length estimate, a new technique termed Q-FISH has been invented. Q-FISH provides estimate of telomere length in each individual chromosome with the resolution of 200 base pairs. In addition, Q-FISH may be used to estimate telomere length in species containing interstitial telomeric sites in their genomes. The classical methodology is non-informative in these cases. Finally, Q-FISH has been essential in estimating telomere length in the mouse, a species with ultra-long telomeres difficult to measure using classical methods. Principles of Q-FISH and its applications are briefly described in this article.
机译：端粒是参与基因组稳定性维持的真核染色体的基本功能元件。端粒功能正确的最重要指标是端粒长度维持在每种物种的典型范围内。端粒长度可以通过基于Southern印迹的经典方法来估计。但是，这种方法相对粗糙，只能提供平均端粒长度的估计值。为了克服传统端粒长度估计的缺点，发明了一种称为Q-FISH的新技术。 Q-FISH提供了每个染色体上端粒长度的估计，分辨率为200个碱基对。另外，Q-FISH可用于估计在其基因组中包含间隙性端粒位点的物种的端粒长度。在这些情况下，经典方法是无用的。最后，Q-FISH在估计小鼠端粒长度方面至关重要，该端粒是具有超长端粒的物种，使用传统方法难以测量。本文简要介绍了Q-FISH的原理及其应用。

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