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首页> 外文期刊>Microbiological Research >Diversity of the ectoines biosynthesis genes in the salt tolerant Streptomyces and evidence for inductive effect of ectoines on their accumulation
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Diversity of the ectoines biosynthesis genes in the salt tolerant Streptomyces and evidence for inductive effect of ectoines on their accumulation

机译：耐盐链霉菌中ectoines生物合成基因的多样性以及ectoines对其积累的诱导作用的证据

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Streptomyces commonly produce ectoines as compatible solutes to prevent osmotic stresses. Fine structure of the genes producing ectoine (ectC) and hydroxyectoine (ectD) enzymes in Streptomyces rimosus C-2012 as a slightly halophilic bacterium is reported in this study. Deduced amino acid sequences of ectC and ectD genes from strain C-2012 and some other related species were compared and 72-90% and 13-81% identities were detected for ectC and ectD, respectively. High similarity of ectC between closely or distantly related Streptomyces to the strain C-2012 may indicate horizontal transfer of this gene. However, phylogenetic relationships of ectD were correlated with phylogenetic affiliation of the strains. It suggests that the ability of Streptomyces to produce hydroxyectoine has been the result of a vertical transfer event. HPLC analysis showed that strain C-2012 was able to produce ectoine and hydroxyectoine both in the presence and absence of external salinity (up to 0.45 M NaCl). Accordingly, reverse transcription quantitative PCR (RT-qPCR) showed that ectABCD operon in this strain is positively affected by salt. Also, inductive effect of the salt was increased when it was applied with 1 mM of ectoines. Transcription level of ectC was increased 2.7- and 2.9-fold in the medium supplied with salt and ectoine and salt and hydroxyectoine, respectively. The effect of salinity with or without ectoines was more on ectD transcription level than that of ectC. In S. rimosus under salt stress, ectoine and hydroxyectoine biosynthesis primarily depends on the stimulation of ectABCD operon transcription. However, drastic accumulation of ectoine and hydroxyectoine without increase in ectC and ectD transcripts was observed in the medium supplied with salt and ectoines and that suggest there might be additional posttranscriptional level of control. Increases in ratio of some intracellular free amino acids in salt stressed to unstressed conditions were observed in cells grown with ectoines. Our results suggest the possibility of a supplementary role of ectoines to improve structure and function of the cells in stressful environments as well as their important role as osmoprotectants
机译：链霉菌通常产生油桃素作为相容性溶质，以防止渗透压。在这项研究中报道了在链状链霉菌C-2012中作为微嗜盐菌的产生菌素（ectC）和羟基菌素（ectD）酶的基因的精细结构。比较了来自菌株C-2012和其他一些相关物种的ectC和ectD基因的推导氨基酸序列，分别检测到ectC和ectD的72-90％和13-81％的同一性。密切相关的链霉菌与菌株C-2012之间的ectC高度相似可能表明该基因的水平转移。但是，ectD的系统发育关系与菌株的系统发育关系相关。这表明链霉菌产生羟基连接素的能力是垂直转移事件的结果。 HPLC分析表明，菌株C-2012在存在和不存在外部盐度（高达0.45 M NaCl）的情况下均能够产生ectoine和hydroxyectoine。因此，逆转录定量PCR（RT-qPCR）显示该菌株中的ectABCD操纵子受盐的正影响。同样，当将盐与1 mM的果胶一起施用时，其诱导作用也会增强。在供应盐和果胶以及盐和羟基果胶的培养基中，ectC的转录水平分别提高了2.7倍和2.9倍。含或不含胶子的盐度对ectD转录水平的影响大于ectC的影响。在盐胁迫下的线虫链球菌中，油桃素和羟基油桃素的生物合成主要取决于对ectABCD操纵子转录的刺激。但是，在添加了盐和ectoines的培养基中观察到了ectoine和hydroxyectoine的大量积累，而ectC和ectD转录本却没有增加，这表明转录后的控制水平可能更高。在用外泌素生长的细胞中，观察到盐对未胁迫条件下某些细胞内游离氨基酸的比例增加。我们的研究结果表明，在压力环境中，油桃素具有补充作用，可以改善细胞的结构和功能，以及它们作为渗透保护剂的重要作用

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