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首页> 外文期刊>Microbiology >Induction and Carbon Catabolite Repression of Isoamylase Production in Rhizopus oryzae PR7~1
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Induction and Carbon Catabolite Repression of Isoamylase Production in Rhizopus oryzae PR7~1

机译：米根霉PR7〜1异淀粉酶产生的诱导和碳分解代谢抑制

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摘要

The growth and the extracellular isoamylase production by Rhizopus oryzae PR7 MTCC 9642 were studied in a stationary culture at 28°C, with maximum isoamylase production obtained after 72 hours. Glycogen was found to be the best inducer for isoamylase synthesis, followed by maltose and dextrin. The enzyme was found to be repressed by glucose and this repression was not overcome by the addition of cGMP. The abrupt reduction in enzyme synthesis after the addition of exogenous glucose in a glycogen-induced culture medium confirmed the repressive action of glucose. An almost similar rate of repression was found to be exerted by α- and β-cyclodextrins. The inhibition of enzyme production after the addition of cycloheximide, a translation blocker, indicated the existence of de novo synthesis of the enzyme.
机译：在28℃的固定培养中研究了米根霉PR7 MTCC 9642的生长和胞外异淀粉酶的产生，在72小时后获得了最大的异淀粉酶的产生。发现糖原是异淀粉酶合成的最佳诱导剂，其次是麦芽糖和糊精。发现该酶被葡萄糖抑制，并且这种抑制不能通过添加cGMP来克服。在糖原诱导的培养基中添加外源葡萄糖后，酶的合成突然减少，证实了葡萄糖的抑制作用。发现α-和β-环糊精的抑制率几乎相似。加入环己酰亚胺（一种翻译阻滞剂）后，对酶产生的抑制作用表明存在该酶的从头合成。

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