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首页> 外文期刊>Microchemical Journal: Devoted to the Application of Microtechniques in all Branches of Science >Determination of calcium, iron and magnesium in rabbit arteries by inductively coupled plasma atomic emission spectrometry
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Determination of calcium, iron and magnesium in rabbit arteries by inductively coupled plasma atomic emission spectrometry

机译：电感耦合等离子体原子发射光谱法测定家兔动脉中钙，铁和镁

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摘要

Regional variations in arterial concentrations of Ca, Mg and Fe may influence susceptibility to atherosclerosis. However, investigation of such hypotheses requires the availability of a sensitive, reliable method for the determination of elements in small arterial samples. These biologically important elements are determined in rabbit arteries by inductively coupled plasma atomic emission spectrometry (ICP-AES). Arterial samples (aorta and iliac arteries) are collected from 4- and 6- to 7-month-old rabbits fed rabbit chow. Closed-vessel microwave acid digestion is used to prepare the samples. The accuracy of the method is tested with a NIST bovine liver (1577b) standard reference material, and the amount of each metal found is within the reported uncertainty in the certified concentration. Also, the recovery from artery samples spiked with 0.5 mug of each metal is nearly 100% (96-105% Ca, 93-105% Fe, and 92-104% Mg). The simultaneous multielement detection of Ca, Fe and Mg at levels more than 1000-fold higher than the detection limit, in arterial samples weighing as little as 5 mg, suggests that this method may be applicable to very small clinical samples or arterial samples from very small animals. (C) 2002 Elsevier Science B.V. All rights reserved. [References: 46]
机译：Ca，Mg和Fe的动脉浓度区域变化可能会影响对动脉粥样硬化的敏感性。然而，对这些假设的研究需要一种灵敏，可靠的方法来确定小动脉样本中的元素。这些生物学上重要的元素是通过感应耦合等离子体原子发射光谱法（ICP-AES）在兔动脉中测定的。从喂养了兔粮的4和6至7个月大的兔子中收集动脉样本（主动脉和动脉）。密闭容器微波酸消解用于制备样品。使用NIST牛肝（1577b）标准参考材料测试该方法的准确性，发现的每种金属的含量在报告浓度的报告不确定度范围内。同样，从掺有0.5杯每种金属的动脉样本中回收的回收率几乎为100％（96-105％的Ca，93-105％的Fe和92-104％的Mg）。在重达5 mg的动脉样本中同时进行Ca，Fe和Mg的多元素检测，其含量比检测限高出1000倍以上，这表明该方法可能适用于非常小的临床样本或来自非常少量的动脉样本小动物。 （C）2002 Elsevier Science B.V.保留所有权利。 [参考：46]

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