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首页> 外文期刊>Microbial Pathogenesis >Polarisation of type III translocation by Pseudomonas aeruginosa requires PcrG, PcrV and PopN
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Polarisation of type III translocation by Pseudomonas aeruginosa requires PcrG, PcrV and PopN

机译：铜绿假单胞菌对III型易位的极化需要PcrG，PcrV和PopN

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摘要

Type III secretion (TTS) mediated translocation of exoenzymes is a key virulence strategy utilised by the opportunistic pathogen Pseudomonas aeruginosa to deliver exoenzyme effectors into the eukaryotic cell. We have previously shown that type III mediated translocation is a contact dependent process, which requires the secreted translocator proteins PerV, PopB and PopD. To further analyse this mechanism, HeLa cells were infected with the wild-type strain PAK as well as isogenic pcrV, popB, popD, pcrG and popN mutants. In the presence of eukaryotic cells, expression of exoenzyme S (ExoS) increased. When cells were infected with the wild-type strain PAK no ExoS was detected in the tissue culture medium. This confirms that ExoS translocation by P. aeruginosa occurs by a polarised mechanism. In contrast, high levels of ExoS were recovered in the tissue Culture medium when cells were infected with pcrG, pcrV and popN mutants. Additionally, ExoS expression levels were higher for these mutants regardless of inducing conditions. This suggests that PcrG, PcrV and PopN are involved in negative regulation of ExoS expression and secretion, and are required to ensure polarised delivery of effectors into target cells. Copyright 2004 Elsevier Ltd. All rights reserved.
机译：III型分泌（TTS）介导的外切酶易位是机会致病性铜绿假单胞菌将外切酶效应子传递到真核细胞中的关键毒力策略。先前我们已经表明，III型介导的易位是接触依赖性过程，需要分泌的易位蛋白PerV，PopB和PopD。为了进一步分析该机制，将野生型菌株PAK以及同基因pcrV，popB，popD，pcrG和popN突变体感染了HeLa细胞。在真核细胞存在下，外切酶S（ExoS）的表达增加。当用野生型PAK感染细胞时，在组织培养基中未检测到ExoS。这证实了铜绿假单胞菌的ExoS易位是通过极化机制发生的。相反，当细胞被pcrG，pcrV和popN突变体感染时，在组织培养基中回收到高水平的ExoS。另外，不管诱导条件如何，这些突变体的ExoS表达水平更高。这表明PcrG，PcrV和PopN参与了ExoS表达和分泌的负调控，并且是确保效应子极化递送至靶细胞所必需的。版权所有2004 ElsevierLtd。保留所有权利。

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