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首页> 外文期刊>Microbial Pathogenesis >Differential expression of sigE by Mycobacterium tuberculosis duringintracellular growth
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Differential expression of sigE by Mycobacterium tuberculosis duringintracellular growth

机译：结核分枝杆菌胞内生长过程中sigE的差异表达

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摘要

The Mycobacterium tuberculosis sigE gene encodes a sigma factor that is a member of the extracytoplasmic function subfamily of sigma factors. Using RT-PCR we demonstrated that sigE is expressed in M. tuberculosis bacilli during growth in human macrophages beginning after 30 min but before 6 h after infection through at least 5 days after infection, but that sigE is not expressed by M. tuberculosis bacteria during growth in Middlebrook 7H9 broth medium. However, sigE expression can be induced by treatment of broth cultures with hydrogen peroxide. Further, sigE is not expressed by M. tuberculosis bacilli during attachment or growth in type II pneumocytes. Using a green fluorescent protein (GFP) reporter gene fused to the sigE promoter, we observed induction of GFP expression following macrophage infection. Western blotting confirmed that sigE protein expression correlated with mRNA expression in induced systems. Analysis of the region of the M. tuberculosis genome encoding sigE suggested it is part of an operon consisting of sigE-orf1-htrA-orf2. The data presented in this report showed that sigE is differentially expressed by M. tuberculosis bacilli in macrophages and might play a role in the pathogenesis of this organism.
机译：结核分枝杆菌sigE基因编码一个西格玛因子，它是西格玛因子的胞外功能亚家族的成员。使用RT-PCR，我们证明了sigE在人巨噬细胞生长期间在感染后至少5天至感染后至少5天的30分钟内开始在人巨噬细胞中表达，但是在感染过程中至少有sigE在结核分枝杆菌中不表达在Middlebrook 7H9肉汤培养基中生长。然而，可以通过用过氧化氢处理肉汤培养物来诱导sigE表达。此外，在II型肺细胞附着或生长期间，结核分枝杆菌不表达sigE。使用融合到sigE启动子的绿色荧光蛋白（GFP）报告基因，我们观察到巨噬细胞感染后GFP表达的诱导。 Western印迹证实在诱导系统中sigE蛋白表达与mRNA表达相关。对编码sigE的结核分枝杆菌基因组区域的分析表明，它是由sigE-orf1-htrA-orf2组成的操纵子的一部分。该报告中提供的数据表明，sigE由结核分枝杆菌在巨噬细胞中差异表达，并可能在这种生物的发病机理中起作用。

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