Soluble Tankyrase Located in Cytosol of Human Embryonic Kidney Cell Line 293

A.N.Kuimov; S.M.Terekhov

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Soluble Tankyrase Located in Cytosol of Human Embryonic Kidney Cell Line 293

机译：可溶性tankyrase位于人类胚胎肾细胞系293细胞质中。

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We studied the subcellular localization of trankyrase in primary and immortalized human cell cultures. In embryonic kidney cell line 293 the enzyme was excluded from the nuclei and distributed in fractions of soluble cytosolic proteins and low-density microsomes. Newly revealed cytosolic tankyrase in its poly (ADP-ribosyl) ated form was passed throught a Sepharose 2B column and eluted as an apparently monomeric protein. The cytosolic localization of the enzyme correlated with its relatively high activity in the 293 cell line in comparison to eight other studied cell types.

机译：我们研究了在原代和永生化的人类细胞培养物中，曲霉酶的亚细胞定位。在胚胎肾细胞系293中，该酶从细胞核中排除，并分布在可溶性胞质蛋白和低密度微粒体中。新发现的聚（ADP-核糖基）化形式的胞质tankyrase通过Sepharose 2B色谱柱洗脱，并以明显的单体蛋白形式洗脱。与其他八种研究细胞类型相比，该酶在293细胞系中的胞质定位与其相对较高的活性有关。

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《Biochemistry》 |2003年第3期|共9页
作者
A.N.Kuimov; S.M.Terekhov;
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正文语种 eng
中图分类生物化学;
关键词
tankyrase; poly (ADP-ribose); ADP-ribosylation;

机译：端锚聚合酶;聚（ADP-核糖）;ADP-核糖基化;

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1. Soluble Tankyrase Located in Cytosol of Human Embryonic Kidney Cell Line 293 [J] . A.N.Kuimov, S.M.Terekhov Biochemistry . 2003,第3期

机译：可溶性tankyrase位于人类胚胎肾细胞系293细胞质中。
2. Overexpressing human membrane proteins in stably transfected and clonal human embryonic kidney 293S cells [J] . Chaudhary S., Pak J.E., Gruswitz F., Nature protocols erecipes for researchers . 2012,第3期

机译：在稳定转染和克隆的人胚胎肾脏293S细胞中过表达人膜蛋白
3. Efficient expression screening of human membrane proteins in transiently transfected Human Embryonic Kidney 293S cells [J] . ChaudharyS., PakJ.E., PedersenB.P., Methods: A Companion to Methods in Enzymology . 2011,第4期

机译：瞬时转染人胚肾293S细胞中人膜蛋白的高效表达筛选
4. Effect of Different Materials Used in Bioreactor Equipments on Cell Growth of Human Embryonic Kidney (HEK293) Cells Cultivated in a Protein-free Medium [C] . Peter Aizawa, Goran Karlsson, Charlotte Benemar, General Meeting of European Society of Animal Cell Technology . 2007

机译：生物反应器设备对蛋白质 - 无胚胎肾（HEK293）细胞细胞生长的不同材料的影响
5. Human kidney embryonic cells on synthetic diamond surfaces. [D] . Baek, SeungWoo. 2014

机译：人造钻石表面上的人类肾脏胚胎细胞。
6. Overexpressing Human Membrane Proteins in Stably Transfected and Clonal Human Embryonic Kidney 293S Cells [O] . Sarika Chaudhary, John E. Pak, Franz Gruswitz, -1

机译：表达人膜蛋白在稳定转染和克隆人类胚胎肾细胞293s
7. Efficient expression screening of human membrane proteins in transiently transfected Human Embryonic Kidney 293S cells [O] . Sarika Chaudhary, John E. Pak, Bjørn P. Pedersen, 2011

机译：瞬时转染人胚胎肾293S细胞中人膜蛋白的高效表达筛选
8. Investigation of the Stoichiometry of Na+ Cotransport with Dopamine in Rat and Human Dopamine Transporters Expressed in Human Embryonic Kidney Cells [R] . Schumacher, P. D. 2001

机译：多巴胺对人胚肾细胞表达大鼠和人多巴胺转运体Na +协同转运的化学计量学研究

Soluble Tankyrase Located in Cytosol of Human Embryonic Kidney Cell Line 293

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