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首页> 外文期刊>Cancer investigation >Upregulation of GRP78 and GRP94 and its function in chemotherapy resistance to VP-16 in human lung cancer cell line SK-MES-1.
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Upregulation of GRP78 and GRP94 and its function in chemotherapy resistance to VP-16 in human lung cancer cell line SK-MES-1.

机译:GRP78和GRP94的上调及其在人肺癌细胞SK-MES-1对VP-16的化疗耐药中的作用。

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摘要

The upregulation of GRP78 and GRP94 under the induction of A23187 and its function in drug resistance to etoposide (VP-16) was investigated in human lung cancer cell line SK-MES-1. The expression of GRP78 and GRP94 induced by A23187 at different concentrations was analyzed by RT-PCR and Western blotting. Cell survival to VP-16 was determined using a colony-formation assay. The expression of GRP78 and GRP94 in the cells was found to correspond well with the cell survival to VP-16. The results showed that upregulation of GRP78 and GRP94 can significantly confer the chemoresistance to VP-16 in human lung cancer cell line SK-MES-1.
机译:在人肺癌细胞SK-MES-1中研究了在A23187的诱导下GRP78和GRP94的上调及其在对依托泊苷(VP-16)的耐药性中的作用。通过RT-PCR和Western印迹分析A23187诱导的不同浓度的GRP78和GRP94的表达。使用集落形成测定法测定到VP-16的细胞存活率。发现细胞中GRP78和GRP94的表达与VP-16的细胞存活很好地对应。结果表明,GRP78和GRP94的上调可以显着赋予人肺癌细胞SK-MES-1 VP-16的化学耐药性。

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